製品の概要

  • 製品名Anti-Villin antibody [EPR3490]
    Villin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3490] to Villin
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human Villin.

  • ポジティブ・コントロール
    • Human small intestine, HT-29 and Caco-2 cell lysates; HT-29 cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 93 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC,IHC-P or IP.
  • ターゲット情報

    • 機能Ca(2+)-regulated actin-binding protein.
    • 組織特異性Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
    • 配列類似性Belongs to the villin/gelsolin family.
      Contains 6 gelsolin-like repeats.
      Contains 1 HP (headpiece) domain.
    • ドメインConsists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
    • 細胞内局在Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 参照データベース
    • 別名
      • D2S1471 antibody
      • OTTHUMP00000164145 antibody
      • VIL antibody
      • VIL1 antibody
      • VILI_HUMAN antibody
      • Villin 1 antibody
      • Villin-1 antibody
      • Villin1 antibody
      see all

    Anti-Villin antibody [EPR3490] 画像

    • All lanes : Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilution

      Lane 1 : Human small intestine lysate
      Lane 2 : HT-29 lysate
      Lane 3 : Caco-2 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 93 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of Villin in permeabilized HT-29 cells, using ab109515 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).

    Anti-Villin antibody [EPR3490] (ab109516) 使用論文

    This product has been referenced in:
    • Negoro R  et al. Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells. Biochem Biophys Res Commun 472:631-6 (2016). Flow Cyt ; Human . Read more (PubMed: 26966071) »
    • Ozawa T  et al. Generation of enterocyte-like cells from human induced pluripotent stem cells for drug absorption and metabolism studies in human small intestine. Sci Rep 5:16479 (2015). Flow Cyt ; Human . Read more (PubMed: 26559489) »

    See all 2 Publications for this product

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    With many products cross reactivity with mouse and rat is determined by western blot only (this is the case for ab109516). If we have only determined the cross reactivity via WB, we will state this information ...

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    Sorry for the confusion. I just wanted to confirm with you that this replacement order, is indeed a free of charge replacement. When the order was first placed the "100%" discount was not taken off the order...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

    Estimated delivery date of this replacement vial is August 27 2012. To ch...

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    Thank you very much for your interest in ab109516. Unfortunately we would not advise that ab109516 be used in ICC. However we have another antibody in our catalogue that may be suitable for your customer. Anti-Villin antibody (ab97512) is a rab...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"