製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Villin
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 7-256 of Human Villin (NP_009058).

  • ポジティブ・コントロール
    • HepG2 whole cell lysate, A549 xenograft

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab97512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 93 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能
    Ca(2+)-regulated actin-binding protein.
  • 組織特異性
    Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
  • 配列類似性
    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
    Contains 1 HP (headpiece) domain.
  • ドメイン
    Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
  • 細胞内局在
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D2S1471 antibody
    • OTTHUMP00000164145 antibody
    • VIL antibody
    • VIL1 antibody
    • VILI_HUMAN antibody
    • Villin 1 antibody
    • Villin-1 antibody
    • Villin1 antibody
    see all

画像

  • Anti-Villin antibody (ab97512) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 93 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of Villin in paraffin embedded A549 xenograft, using ab97512 at a 1/500 dilution.

参考文献

ab97512 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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