Anti-VGLUT3 抗体 [N34/34] (ab134310)
Key features and details
- Mouse monoclonal [N34/34] to VGLUT3
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-VGLUT3 antibody [N34/34]
VGLUT3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [N34/34] to VGLUT3 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, IHC-Fr, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment (His-tag) within Rat VGLUT3 aa 500 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q7TSF2 -
ポジティブ・コントロール
- ICC/IF: SK-N-BE cells.
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特記事項
The clone number has been updated from S34-34 to N34/34, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N34/34 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab134310の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration.
|
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IHC-P | (1) |
Use at an assay dependent concentration.
|
IHC-Fr |
Use at an assay dependent concentration.
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|
ICC/IF |
1/100.
|
特記事項 |
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WB
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
1/100. |
ターゲット情報
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機能
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. -
組織特異性
Expressed in amygdala, cerebellum, hippocampus, medulla, spinal cord and thalamus. -
関連疾患
Defects in SLC17A8 are the cause of deafness autosomal dominant type 25 (DFNA25) [MIM:605583]. DFNA25 is a form of sensorineural hearing loss. The expression of DFNA25 deafness is variable in terms of onset and rate of progression, with an age-dependent penetrance resembling an early-onset presbycusis, or senile deafness, a progressive bilateral loss of hearing that occurs in the aged. -
配列類似性
Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. -
細胞内局在
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Membrane. Cell junction > synapse > synaptosome. - Information by UniProt
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参照データベース
- Entrez Gene: 246213 Human
- Entrez Gene: 216227 Mouse
- Entrez Gene: 266767 Rat
- Omim: 607557 Human
- SwissProt: Q8NDX2 Human
- SwissProt: Q8BFU8 Mouse
- SwissProt: Q7TSF2 Rat
- Unigene: 116871 Human
see all -
別名
- deafness autosomal dominant 25 antibody
- DFNA 25 antibody
- DFNA25 antibody
see all
画像
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Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (Human neuroblastoma cell line) cells labeling VGLUT3 using ab134310 at 1/100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min at RT, 5 min at RT.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab134310 は 1 報の論文で使用されています。
- Zhang XL et al. Reorganization of postmitotic neuronal chromatin accessibility for maturation of serotonergic identity. Elife 11:N/A (2022). PubMed: 35471146