製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to VGluT1
  • 特異性
    Due to immunogen homology, the antibody may detect VGluT2 (100%) and VGluT3(95%).
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human VGLUT1.

    (Peptide available as ab95408.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human tissue lysates: Liver; Kidney.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85922 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 50 kDa).

ターゲット情報

  • 機能
    Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.
  • 組織特異性
    Expressed in several regions of the brain including amygdala, cerebellum, cerebral cortex, hippocampus, frontal lobe, medulla, occipital lobe, putamen and temporal lobe.
  • 配列類似性
    Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily.
  • 細胞内局在
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BNPI antibody
    • Brain specific Na (+) dependent inorganic phosphate cotransporter antibody
    • Brain specific Na dependent inorganic phosphate cotransporter antibody
    • Brain-specific Na(+)-dependent inorganic phosphate cotransporter antibody
    • Slc17a7 antibody
    • Solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7 antibody
    • Solute carrier family 17 member 7 antibody
    • Vesicular glutamate transporter 1 antibody
    • VGLU1_HUMAN antibody
    • VGluT1 antibody
    see all

画像

  • All lanes : Anti-VGluT1 antibody (ab85922) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

プロトコール

参考文献

ab85922 has not yet been referenced specifically in any publications.

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