製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to VGF
  • アプリケーション
    適用あり: ICC/IF, WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide derived from a internal sequence of human VGF.

  • ポジティブ・コントロール
    • Human lung carcinoma. Extracts from RAW264.7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74140 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 67 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
    NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
  • 組織特異性
    Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
  • 翻訳後修飾
    Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
  • 細胞内局在
    Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • NERP-1 antibody
    • NERP-2 antibody
    • Nerve growth factor inducible antibody
    • Neuroendocrine regulatory peptide-2 antibody
    • Neurosecretory protein VGF antibody
    • VGF antibody
    • VGF_HUMAN antibody
    see all

画像

  • All lanes : Anti-VGF antibody (ab74140) at 1/500 dilution

    Lane 1 : Extracts from RAW264.7 cells
    Lane 2 : Extracts from RAW264.7 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • Immunohistochemical analysis of VGF in paraffin embedded human lung carcinoma tissue using ab74140, at a 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • ICC/IF image of ab74140 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74140, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Vahdati Hassani F  et al. Antidepressant effects of crocin and its effects on transcript and protein levels of CREB, BDNF, and VGF in rat hippocampus. Daru 22:16 (2014). WB ; Rat . Read more (PubMed: 24401376) »

See 1 Publication for this product

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