製品の概要

  • 製品名Anti-VGF antibody
    VGF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VGF
  • アプリケーション適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    17 amino acid peptide near the carboxy terminus of the human VGF (GenBank accession no. NP_003369).

  • ポジティブ・コントロール
    • Human brain tissue lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 67 kDa (predicted molecular weight: 67 kDa).

ターゲット情報

  • 機能May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
    NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
  • 組織特異性Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
  • 翻訳後修飾Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
  • 細胞内局在Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • NERP-1 antibody
    • NERP-2 antibody
    • Nerve growth factor inducible antibody
    • Neuroendocrine regulatory peptide-2 antibody
    • Neurosecretory protein VGF antibody
    • VGF antibody
    • VGF_HUMAN antibody
    see all

Anti-VGF antibody 画像

  • Lane 1 : Anti-VGF antibody (ab69989) at 0.5 µg/ml
    Lane 2 : Anti-VGF antibody (ab69989) at 1 µg/ml

    Lane 1 : Human brain tissue lysate
    Lane 2 : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 67 kDa
    Observed band size : 67 kDa
  • ab69989 at 5 µg/ml staining VGF in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).

  • ICC/IF image of ab69989 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69989, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of VGF in Human Brain cells using ab69989 at 20 ug/ml.

Anti-VGF antibody (ab69989) 使用論文

This product has been referenced in:
  • Annaratone L  et al. Search for neuro-endocrine markers (chromogranin A, synaptophysin and VGF) in breast cancers. An integrated approach using immunohistochemistry and gene expression profiling. Endocr Pathol 25:219-28 (2014). WB, IHC-P ; Human . Read more (PubMed: 24277232) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Spermophilus tridecemlineatus Tissue lysate - whole (brain)
Specification brain
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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投稿 Apr 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"