製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to VGF
  • 特異性
    The immunogen is after amino acid 577 of O15240, so the antibody should only cross-react with full-length VGF and none of the processed peptides.
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    17 amino acid peptide near the carboxy terminus of the Human VGF.

  • ポジティブ・コントロール
    • Human Brain Cortex tissue; Human Brain tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system.
    NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
  • 組織特異性
    Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
  • 翻訳後修飾
    Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
  • 細胞内局在
    Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • NERP-1 antibody
    • NERP-2 antibody
    • Nerve growth factor inducible antibody
    • Neuroendocrine regulatory peptide-2 antibody
    • Neurosecretory protein VGF antibody
    • VGF antibody
    • VGF_HUMAN antibody
    see all

画像

  • ab115609, at 5µg/ml, staining VGF in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Lane 1 : Anti-VGF antibody (ab115609) at 0.5 µg/ml
    Lane 2 : Anti-VGF antibody (ab115609) at 1 µg/ml

    Lane 1 : Human Brain tissue lysate
    Lane 2 : Human Brain tissue lysate


    Predicted band size : 67 kDa

参考文献

ab115609 has not yet been referenced specifically in any publications.

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