The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 67 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May be involved in the regulation of cell-cell interactions or in synatogenesis during the maturation of the nervous system. NERP peptides are involved in the control of body fluid homeostasis by regulating vasopressin release.
Central and peripheral nervous systems, synthesized exclusively in neuronal and neuroendocrine cells.
Multiple peptides are derived from VGF, with activities in synaptic plasticity, antidepression, penile erection, autonomic activation, and increases in energy expenditure.
Secreted. Cytoplasmic vesicle > secretory vesicle. Stored in secretory vesicles and then secreted, NERP peptides colocalize with vasopressin in the storage granules of hypothalamus.
ab115609, at 5µg/ml, staining VGF in Formalin-fixed, Paraffin-embedded Human Brain cortex tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-VGF antibody (ab115609)
Lane 1 : Anti-VGF antibody (ab115609) at 0.5 µg/ml Lane 2 : Anti-VGF antibody (ab115609) at 1 µg/ml
Lane 1 : Human Brain tissue lysate Lane 2 : Human Brain tissue lysate