Anti-Vesicular Acetylcholine Transporter 抗体 (ab31544)

製品の概要

  • 製品名Anti-Vesicular Acetylcholine Transporter antibody
    Vesicular Acetylcholine Transporter 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to Vesicular Acetylcholine Transporter
  • 特異性Ab31544 is specific to vesicular acetylcholine transporter.
  • アプリケーション適用あり: IHC - Wholemount, WBmore details
  • 種交差性
    交差種: Rat, Guinea pig
    交差が予測される動物種: Mouse, Dog, Human, Chimpanzee, Macaque Monkey
  • 免疫原

    Synthetic peptide:

    C-TRSRSERDVLLDEPPQGLYDAVRLRE

    , corresponding to amino acids 475-500 of Rat Vesicular Acetylcholine Transporter conjugated to Blue carrier protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC - Wholemount Use at an assay dependent concentration. Fix Tissue overnight in Zamboni fixative, then carry out 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS and Azide until being dissected and stained.
WB Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.

ターゲット情報

Anti-Vesicular Acetylcholine Transporter antibody 画像

  • Immunohistochemical analysis of Vesicular Acetylcholine Transporter in guinea pig gut.

    Method: Guinea Pig Ileum removed and fixed overnight in Zamboni fixative, then cleared 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS Azide until being dissected and used in this experiment.
    Primary: Anti-Vesicular Acetylcholine Transporter antibody (ab31544) diluted 1:1000 incubated overnight, washed 3 x 10 mins.
    Secondary: antibody incubated for 2 hours washed in PBS, put in 100% Glycerol and mounted.
    Magnification: 40X.

Anti-Vesicular Acetylcholine Transporter antibody (ab31544) 使用論文

ab31544 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Spinal cord)
Antigen retrieval step None
Permeabilization Yes - 0.1% Tween 20
Specification Spinal cord
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
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Mrs. Teresa Garrett

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投稿 Oct 14 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"