Anti-USO1 抗体 (ab102470)
Key features and details
- Rabbit polyclonal to USO1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-USO1 antibody
USO1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to USO1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to Human USO1 aa 36-177.
Database link: AAH3265 -
ポジティブ・コントロール
- A431, 293T, Jurkat and Raji cell lysates; Lung Adenocarcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab102470の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (4) |
Use at an assay dependent concentration.
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WB | (3) |
1/5000 - 1/20000. Predicted molecular weight: 108 kDa.
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IHC-P |
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0).
|
特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/5000 - 1/20000. Predicted molecular weight: 108 kDa. |
IHC-P
1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0). |
ターゲット情報
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機能
General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. -
配列類似性
Belongs to the VDP/USO1/EDE1 family.
Contains 10 ARM repeats. -
ドメイン
Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain. -
翻訳後修飾
Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation. -
細胞内局在
Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 317724 Cow
- Entrez Gene: 8615 Human
- Entrez Gene: 56041 Mouse
- Entrez Gene: 56042 Rat
- Omim: 603344 Human
- SwissProt: P41541 Cow
- SwissProt: O60763 Human
- SwissProt: Q9Z1Z0 Mouse
see all -
別名
- General vesicular transport factor antibody
- General vesicular transport factor p115 antibody
- P115 antibody
see all
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab102470 は 3 報の論文で使用されています。
- McCormick R et al. Optimisation of a Novel Bio-Substrate as a Treatment for Atrophic Age-Related Macular Degeneration. Front Bioeng Biotechnol 8:456 (2020). PubMed: 32500067
- Sen P et al. Identification of p115 as a novel ACSL4 interacting protein and its role in regulating ACSL4 degradation. J Proteomics 229:103926 (2020). PubMed: 32736139
- Howley BV et al. A CREB3-regulated ER-Golgi trafficking signature promotes metastatic progression in breast cancer. Oncogene 37:1308-1325 (2018). PubMed: 29249802