Anti-Vesicle docking protein p115 抗体 (ab102470)

製品の概要

  • 製品名Anti-Vesicle docking protein p115 antibody
    Vesicle docking protein p115 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Vesicle docking protein p115
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 36 and 177 of Human Vesicle docking protein p115 (AAH32654).

  • ポジティブ・コントロール
    • A431, 293T, Jurkat and Raji cell lysates; Lung Adenocarcinoma tissue

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab102470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB 1/5000 - 1/20000. Predicted molecular weight: 108 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0).

ターゲット情報

  • 機能General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
  • 配列類似性Belongs to the VDP/USO1/EDE1 family.
    Contains 10 ARM repeats.
  • ドメインComposed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.
  • 翻訳後修飾Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.
  • 細胞内局在Cytoplasm > cytosol. Golgi apparatus membrane. Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • General vesicular transport factor antibody
    • General vesicular transport factor p115 antibody
    • P115 antibody
    • Protein USO1 homolog antibody
    • TAP antibody
    • Transcytosis associated protein antibody
    • Transcytosis-associated protein antibody
    • Uso1 antibody
    • USO1_HUMAN antibody
    • VDP antibody
    • Vesicle docking protein antibody
    • Vesicle-docking protein antibody
    see all

Anti-Vesicle docking protein p115 antibody 画像

  • Anti-Vesicle docking protein p115 antibody (ab102470) at 1/10000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 108 kDa
    7.5% SDS-PAGE.
  • ab102470 at 1/500 dilution staining Vesicle docking protein p115 in Human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
  • ab102470 staining Vesicle docking protein p115 in human HeLa cells using ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% donkey serum in 0.1% PBST for 1 hour at 22oC. Samples were incubated with primary antibody (1/50 in 1% donkey serum in 0.1% PBST) for 3 hours at 22oC. Alexa Fluor® 594 conjugated Donkey-anti-Rb polyclonal (1/200) was used as the secondary antibody.

Anti-Vesicle docking protein p115 antibody (ab102470) 使用論文

ab102470 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Loading amount 20 µg
Specification Rat hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jan 20 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cells)
Permeabilization Yes - 0.2% Triton X-100
Specification HeLa cells
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 19 2015

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (4-15% gradient SDS-PAGE)
Sample Human Cell lysate - whole cell (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Nov 14 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Panc-1)
Specification Panc-1
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Petrosyan

Verified customer

投稿 Apr 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"