Anti-VEGFD 抗体 [EPR8457] (ab155288)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8457] to VEGFD
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-VEGFD antibody [EPR8457]
VEGFD 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8457] to VEGFD -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
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ポジティブ・コントロール
- Raji, HT-1080, MCF-7, and Caco-2 cell lysates. Human heart and lung tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8457 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155288の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/100 - 1/700. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/200.
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特記事項 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
IHC-P
1/100 - 1/700. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/200. |
ターゲット情報
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機能
Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. -
組織特異性
Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas. -
配列類似性
Belongs to the PDGF/VEGF growth factor family. -
翻訳後修飾
Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 2277 Human
- Entrez Gene: 14205 Mouse
- Entrez Gene: 360457 Rat
- Omim: 300091 Human
- SwissProt: O43915 Human
- SwissProt: P97946 Mouse
- SwissProt: O35251 Rat
- Unigene: 11392 Human
see all -
別名
- c-fos induced growth factor antibody
- c-fos induced growth factor (vascular endothelial growth factor D) antibody
- c-fos induced growth factor antibody
see all
画像
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ab155288 staining VEGFD in human cardiac muscle tissue sections by Immunohistochemistry (IHC-P - Formalin/paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control: PBS in place of primary antibody.
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ab155288 staining VEGFD in MCF-7 (human breast carcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab155288 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) -
All lanes : Anti-VEGFD antibody [EPR8457] (ab155288) at 1/10000 dilution
Lane 1 : Raji (human Burkitt's lymphoma) whole cell lysate
Lane 2 : HT1080 (human fibrosarcoma) whole cell lysate
Lane 3 : Caco-2 (human colorectal adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.Diluting and blocking buffer: 5% NFDM/TBST
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Anti-VEGFD antibody [EPR8457] (ab155288) at 1/10000 dilution (Unpurified) + GST-Tagged human VEGFD (aa 1 to 354) recombinant protein
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM/TBST
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ab155288 staining VEGFD in the mouse cell line NIH/3T3 (mouse embryo) by intracellular flow cytometry.Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/200. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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All lanes : Anti-VEGFD antibody [EPR8457] (ab155288) at 1/2000 dilution
Lane 1 : Rat lung tissue lysate
Lane 2 : NIH/3T3 (mouse embryo) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.Diluting and blocking buffer: 5% NFDM/TBST
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All lanes : Anti-VEGFD antibody [EPR8457] (ab155288) at 1/1000 dilution
Lane 1 : Raji lysate
Lane 2 : HT-1080 lysate
Lane 3 : MCF-7 lysate
Lane 4 : Caco-2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
Immunohistochemical analysis of paraffin embedded Human heart tissue labeling VEGFD with ab155288 at a 1/100 dilution.
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Immunohistochemical analysis of paraffin embedded Human lung tissue labeling VEGFD with ab155288 at a 1/100 dilution.
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Immunohistochemical analysis of paraffin embedded Human normal kidney tissue using ab155288 showing +ve staining.
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Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue using ab155288showing +ve staining.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab155288 は 15 報の論文で使用されています。
- Kang CM et al. Distinctive cytokine profiles of stem cells from human exfoliated deciduous teeth and dental pulp stem cells. J Dent Sci 17:276-283 (2022). PubMed: 35028048
- Yen JH et al. Improved Wound Healing by Naringin Associated with MMP and the VEGF Pathway. Molecules 27:N/A (2022). PubMed: 35268795
- Liu P et al. Vasohibin 2 promotes lymphangiogenesis of lung squamous cell carcinoma through snail-dependent vascular endothelial growth factor-D (VEGF-D) signaling pathway. Ann Transl Med 10:39 (2022). PubMed: 35282047
- Zhou Z et al. Depletion of PARP10 inhibits the growth and metastatic potential of oral squamous cell carcinoma. Front Genet 13:1035638 (2022). PubMed: 36313419
- Yang X et al. Expression of Ubiquitin-specific protease 7 in oral squamous cell carcinoma promotes tumor cell proliferation and invasion. Genet Mol Biol 44:e20210058 (2021). PubMed: 34812471