製品の概要

  • 製品名Anti-VEGFD antibody
    VEGFD 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VEGFD
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Macaque Monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human VEGFD.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HT1080 whole cell lysate as well as the following tissue lysates: Human Heart; Human Fetal Lung; Human Colon.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab103685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IHC-P Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
  • 組織特異性Highly expressed in lung, heart, small intestine and fetal lung, and at lower levels in skeletal muscle, colon, and pancreas.
  • 配列類似性Belongs to the PDGF/VEGF growth factor family.
  • 翻訳後修飾Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3 and VEGFR-2. VEGF-D first form an antiparallel homodimer linked by disulfide bonds before secretion. The fully processed VEGF-D is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c-fos induced growth factor antibody
    • c-fos induced growth factor (vascular endothelial growth factor D) antibody
    • c-fos induced growth factor antibody
    • c-fos-induced growth factor antibody
    • FIGF antibody
    • Vascular endothelial growth factor D antibody
    • Vascular endothelial growth factor D precursor antibody
    • Vascular endothelial growth factor D precursor antibody
    • VEGF D antibody
    • VEGF-D antibody
    • VEGFD antibody
    • VEGFD_HUMAN antibody
    see all

Anti-VEGFD antibody 画像

  • All lanes : Anti-VEGFD antibody (ab103685) at 1 µg/ml

    Lane 1 : HT 1080 (Human fibrosarcoma) Whole Cell Lysate
    Lane 2 : Human heart tissue lysate - total protein (ab29431)
    Lane 3 : Human Fetal Heart - Total Protein Tissue Lysate
    Lane 4 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Additional bands at : 140 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • IHC image of VEGFD staining in Human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103685, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-VEGFD antibody (ab103685) 使用論文

ab103685 has not yet been referenced specifically in any publications.

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