Anti-VEGFC 抗体 [mAbcam 63221] (ab63221)

製品の概要

  • 製品名Anti-VEGFC antibody [mAbcam 63221]
    VEGFC 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAbcam 63221] to VEGFC
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human VEGFC.

    (Peptide available as ab97415.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human lysates: Spleen tissue; Lymph Node tissue; Thymus tissue; HepG2 whole cell; HEK293 whole cell. This antibody also detects a band of the expected molecular weight when tested against recombinant VEGFC protein (ab83573).
  • 特記事項

    Alternative versions available:

    Anti-VEGFC antibody (FITC) [mAbcam 63221] (ab150262)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 10 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
  • 組織特異性Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.
  • 配列類似性Belongs to the PDGF/VEGF growth factor family.
  • 翻訳後修飾Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing an heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Flt 4L antibody
    • Flt4 ligand antibody
    • FLT4 ligand DHM antibody
    • Flt4-L antibody
    • Flt4L antibody
    • Vascular endothelial growth factor C antibody
    • Vascular endothelial growth factor related protein antibody
    • Vascular endothelial growth factor-related protein antibody
    • VEGF C antibody
    • VEGF-C antibody
    • Vegfc antibody
    • VEGFC_HUMAN antibody
    • VRP antibody
    see all

Anti-VEGFC antibody [mAbcam 63221] 画像

  • All lanes : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 10 µg/ml

    Lane 1 : Human spleen tissue lysate - total protein (ab29699)
    Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
    Lane 3 : Human thymus tissue lysate - total protein (ab30146)
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 38 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • Lane 1 : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 1 µg/ml
    Lane 2 : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 10 µg/ml

    Lane 1 : Recombinant Human VEGFC protein (ab83573)
    Lane 2 : Recombinant Human VEGFC protein (ab83573)

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 47 kDa
    Observed band size : 47 kDa
    Additional bands at : 38 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Overlay histogram showing HepG2 cells stained with ab63221 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63221, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-VEGFC antibody [mAbcam 63221] (ab63221) 使用論文

ab63221 has not yet been referenced specifically in any publications.

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