Anti-VEGFC 抗体 [mAbcam 63221] (ab63221)

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製品の概要

  • 製品名
    Anti-VEGFC antibody [mAbcam 63221]
    VEGFC 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mAbcam 63221] to VEGFC
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human VEGFC.

    Immunogen の所有権に関して

    (Peptide available as ab97415.)

  • ポジティブ･コントロール
    • This antibody gave a positive signal in the following human lysates: Spleen tissue; Lymph Node tissue; Thymus tissue; HepG2 whole cell; HEK293 whole cell. This antibody also detects a band of the expected molecular weight when tested against recombinant VEGFC protein (ab83573).
  • 特記事項

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 10 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能
    Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
  • 組織特異性
    Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.
  • 配列類似性
    Belongs to the PDGF/VEGF growth factor family.
  • 翻訳後修飾
    Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing an heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Flt 4L antibody
      • Flt4 ligand antibody
      • FLT4 ligand DHM antibody
      • Flt4-L antibody
      • Flt4L antibody
      • Vascular endothelial growth factor C antibody
      • Vascular endothelial growth factor related protein antibody
      • Vascular endothelial growth factor-related protein antibody
      • VEGF C antibody
      • VEGF-C antibody
      • Vegfc antibody
      • VEGFC_HUMAN antibody
      • VRP antibody
      see all

    画像

    • Western blot - Anti-VEGFC antibody [mAbcam 63221] (ab63221)
      Western blot - Anti-VEGFC antibody [mAbcam 63221] (ab63221)
      All lanes : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 10 µg/ml

      Lane 1 : Human spleen tissue lysate - total protein (ab29699)
      Lane 2 : Human lymph node tissue lysate - total protein (ab29871)
      Lane 3 : Human thymus tissue lysate - total protein (ab30146)
      Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 47 kDa
      Observed band size: 47 kDa
      Additional bands at: 38 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute


      In human spleen, lymph node and thymus tissue lysates, this antibody detects a band close to 47-kDa, corresponding to the predicted MW of VEGF-C. In cell lysates, the antibody detects the same 47-kDa, as well as additional bands around 50-kDa and 37-kDa. The target protein can be glycosylated and the sequence contains both a 31 residue signal sequence and two pro-peptides (residues 32-111 and 228-419). VEGF-C undergoes a complex proteolytic maturation which generates a variety of processed secreted forms.   

      Further characterization on this product is underway. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
    • Western blot - Anti-VEGFC antibody [mAbcam 63221] (ab63221)
      Western blot - Anti-VEGFC antibody [mAbcam 63221] (ab63221)
      Lane 1 : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 1 µg/ml
      Lane 2 : Anti-VEGFC antibody [mAbcam 63221] (ab63221) at 10 µg/ml

      All lanes : Recombinant Human VEGFC protein (ab83573)

      Lysates/proteins at 0.1 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 47 kDa
      Observed band size: 47 kDa
      Additional bands at: 38 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes


      Individual chains of ab83573 migrate as a band between 27 and 35 kDa in SDS-PAGE due to post-translational modifications, in particular glycosylation.
    • Flow Cytometry - Anti-VEGFC antibody [mAbcam 63221] (ab63221)
      Flow Cytometry - Anti-VEGFC antibody [mAbcam 63221] (ab63221)
      Overlay histogram showing HepG2 cells stained with ab63221 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab63221, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    参考文献

    ab63221 has not yet been referenced specifically in any publications.

    ab63221 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    2 reviews or Q&A

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    Thanks for reply. Please find enclosed notes to the inquiries you asked and feel free let me know if there is further question.

    Thanks

    1) Sample: Cell extract

    Could you specify the species of the samples and the cell or...

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    Thank you for getting back to me and for answering to my further questions.

    As you may be aware Our policy is that we will offer pre- and post-sale technical support for products sold via unauthorized distributors. In case of problems with ou...

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    Dear Technical, would you please take a look over the attached antibody and advise what you may suggest for the problem.

    Thanks a lot







    Product Information





    Produ...

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    Thank you for your enquiry regarding ab63221 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is having problems with this antibody.

    Though you have kindly provided some det...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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