製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to VEGFC
  • アプリケーション
    適用あり: IHC-FoFr, WB, ELISA, IHC-Pmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Rat, Sheep, Human
  • 免疫原

    Recombinant fragment corresponding to Rat VEGFC aa 101-221. Produced from sera of rabbits pre-immunized with highly pure recombinant rat VEGF-C (Asp101-Ile221) derived from insect cells.
    Sequence:

    DTVKLAAAHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGAATNTFFKP PCVSVYRCGGCCNSEGLQCMNTSTGYLSKTLFEITVPLSQGPKPVTISFA NHTSCRCMSKLDVYRQVHSIIHHHHHH


    Database link: P49767

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 22206010
WB Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 1 - 5 µg/ml.

Allows the detection of 0.5-1.0 ng/well of recombinant rat VEGF-C.

IHC-P Use at an assay dependent concentration. PubMed: 21359148
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors.
    • 組織特異性
      Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte.
    • 配列類似性
      Belongs to the PDGF/VEGF growth factor family.
    • 翻訳後修飾
      Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing an heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions.
    • 細胞内局在
      Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Flt 4L antibody
      • Flt4 ligand antibody
      • FLT4 ligand DHM antibody
      • Flt4-L antibody
      • Flt4L antibody
      • Vascular endothelial growth factor C antibody
      • Vascular endothelial growth factor related protein antibody
      • Vascular endothelial growth factor-related protein antibody
      • VEGF C antibody
      • VEGF-C antibody
      • Vegfc antibody
      • VEGFC_HUMAN antibody
      • VRP antibody
      see all

    Anti-VEGFC antibody 画像

    • Western analysis of recombinant human and rat VEGF-C using a polyclonal rabbit anti-rat VEGF-C antibody

    • Western analysis of recombinant rat VEGF-C using a polyclonal rabbit anti-rat VEGF-C antibody

    • Western blot with human and rat VEGF C and VEGF D using the polyclonal anti VEGF C antibody ab9546. Proteins were separated by 15% SDS-PAGE and blotted on to PVDF membranes as described. After transfer, the membrane was incubated for 1 h with ab9546 at 1 µg/ml in TBS containing 20% non-fat milk followed by incubation with a goat-anti-rabbit alkaline phosphatase-conjugated secondary antibody.  Lane 1/250ng rat VEGF C; lane 2/250 ng human VEGF C; lane 3/250 ng rat VEGF D; lane4/250 ng human VEGF-D.

    • Anti-VEGFC antibody (ab9546) + Rat Recombinant VEGF-C insect cells Western Blot was run on a 15% SDS-PAGE gel. Bands between 15kDa and 20kDa represent the different glycoslation forms.
    • Western blot with recombinant rat VEGF C fused to His-tag using the polyclonal anti VEGF C antibody ab9546. Bands are between 15kDa and 20kDa represents glycosylation forms. 

       

    • VEGF-C sandwich-ELISA using ab9546 as capture antibody and recombinant rat VEGF-C as standard. Biotinylated rabbit anti-rat VEGF-C was used for detection.

    Anti-VEGFC antibody (ab9546) 使用論文

    This product has been referenced in:
    • Mankowska SA  et al. A Shorter Route to Antibody Binders via Quantitative in vitro Bead-Display Screening and Consensus Analysis. Sci Rep 6:36391 (2016). Read more (PubMed: 27819305) »
    • Zhu G  et al. Lipopolysaccharide increases the release of VEGF-C that enhances cell motility and promotes lymphangiogenesis and lymphatic metastasis through the TLR4- NF-?B/JNK pathways in colorectal cancer. Oncotarget 7:73711-73724 (2016). WB ; Human . Read more (PubMed: 27713159) »

    See all 14 Publications for this product

    Product Wall

    Hello! This antibody should be able to detect both the prescursor and mature VEGFC. Please let me know if I can be of further assistance.

    Thank you for contacting us.


    The rabbit anti-mouse VEGF-A antibody ab51745 did not cross react with human VEGF-C in Western blotting (reduced, non-reduced). According to our results the rabbit anti-rat VEGF-C ab9546 cross reacts with huma...

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    Thank you for contacting us.

    I am happy that you have decided to use our antibody for your research purposes. 1 vial of ab9546 will contain 100 ul at 1mg/ml. This will be sufficient for you to perform 10 western blots and 10 immunohistochemis...

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    Thank you for contacting us.

    Firstly I would like to confirm that you are referring to ab9546 and not ab9456?

    ab9546 is a rabbit polyclonal and therefore does not have a specific epitope. This antibody contains a combination of imm...

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    Thank you for contacting us.

    VEGF-C analysis has not yet been done on human skin with antibody ab9546. However, this antibody is guarenteed to work in humans and in WB and IHC therefore it should be fine to use ab9456 on
    innumohistochem...

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    I am very sorry to hear that you have been experiencing problems using this product in the application that you wish.

    I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guaran...

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    Thank you for your reply.

    I was wandering if you had tried using a higher concentration of the antibody? If the antibody binds to the mouse protein with a lower affinity (this is a theory of mine, we have not actually tested it), then you may ...

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    Thank you for your patience while I was looking into this matter.

    I have been searching our notes and I cannot find the reference number for the exact VEGF-C protein that was used to so the test ELISA, but I suspect that it was a protein that ...

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    Thank you for contacting us.

    Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.
    We are happy to reassure our customers that all of our products are covered by our Abpromise, which gu...

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    Following up my previous e-mail, I received the following from our source for this antibody:

    "The proteolytic processing of mammalian VEGF-C is a complicated step and described before. Therefore the work by Joukov et al. (EMBO J 16, pp3898-1...

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    1-10 of 13 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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