Anti-VEGFA 抗体 [SP28], prediluted (ab27620)

製品の概要

  • 製品名Anti-VEGFA antibody [SP28], prediluted
    VEGFA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP28] to VEGFA, prediluted
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human VEGFA (N terminal).

  • ポジティブ・コントロール
    • Tumor cells in hemangiosarcomas

アプリケーション

Our Abpromise guarantee covers the use of ab27620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

ターゲット情報

  • 機能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • 組織特異性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • 関連疾患Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 配列類似性Belongs to the PDGF/VEGF growth factor family.
  • 細胞内局在Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all

Anti-VEGFA antibody [SP28], prediluted 画像

  • Immunohistochemical staining of human angiosarcoma labelling VEGF (SP28) with ab27620 at a 1:1 no dilution.

  • Paraffin embedded formalin fixed human angioscarcoma tissue section stained with prediluted anti VEGF antibody ab27620.

Anti-VEGFA antibody [SP28], prediluted (ab27620) 使用論文

This product has been referenced in:
  • Schaefer IM  et al. Pyloric gland adenoma of the cystic duct with malignant transformation: report of a case with a review of the literature. BMC Cancer 12:570 (2012). Human . Read more (PubMed: 23206236) »
  • Biswas S  et al. Effects of HIF-1alpha and HIF2alpha on Growth and Metabolism of Clear-Cell Renal Cell Carcinoma 786-0 Xenografts. J Oncol 2010:757908 (2010). IHC-P ; Human . Read more (PubMed: 20652061) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate, pH6.0, 20 min at 100C
Sample Human Tissue sections (Osteosarcoma, Breast Carcinoma)
Specification Osteosarcoma, Breast Carcinoma
Permeabilization No
Fixative Formaldehyde
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Abcam user community

Verified customer

投稿 Feb 26 2015

Thank you for contacting us and your interest in our products.


We do not have any experimental data regarding the specificity of the anti-VEGF antibody (ab27620)to the various isoforms of VEGF-A.By sequence homology of the immunogen use...

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Thank you for contacting Abcam regarding ab27620, lot# GR6603-1. Unfortunately, we do not have anymore of lot#GR6603-1 available. If there is anything else I can help you with, please let me know.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (hemangioma)
Specification hemangioma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

投稿 Feb 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"