Anti-VEGFA 抗体 (Biotin) (ab83132)


  • 製品名
    Anti-VEGFA antibody (Biotin)
    VEGFA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VEGFA (Biotin)
  • 標識
  • アプリケーション
    適用あり: ELISA, Sandwich ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein corresponding to Human VEGFA.



Our Abpromise guarantee covers the use of ab83132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human VEGF.
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFA (ab9570). To detect hVEGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 - 1.0 µg/ml of this antibody as Capture is required. This biotinylated polyclonal antibody, paired with the above recommended pair, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant human VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.


  • 機能
    Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • 組織特異性
    Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • 関連疾患
    Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 配列類似性
    Belongs to the PDGF/VEGF growth factor family.
  • 細胞内局在
    Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Folliculostellate cell-derived growth factor antibody
    • Glioma-derived endothelial cell mitogen antibody
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor A121 antibody
    • vascular endothelial growth factor A165 antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all

Anti-VEGFA antibody (Biotin) 画像

  • Detected human VEGF in a sandwich ELISA within the range 16-1000 pg/ml
    - 0.5 µg/ml of capture antibody (ab9570)
    - 0.25 µg/ml of detection antibody (ab83132)


Anti-VEGFA antibody (Biotin) (ab83132) 使用論文

ab83132 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

The antibodies are viable for 10 years when stored at -20C, for 1 year when stored at 4C.

Please note that we recommend storing the antibodies at 4C for 1-2 weeks only. Some antibodies are prone to degrada...

Read More

Merci de votre intérêt pour ab83132 et ab93540.

Comme expliqué par téléphone, à notre connaissance ces deuxanticorps n’ont pas ...

Read More

Thank you for contacting us.

Thefollowing CROSS REACTIVITY has been tested with the antibodies ab9570 and ab83132:

When prepared at 50ng/ml the following antigens exhibited 100% cross reactivity:

Human VEGF121

Read More

Thank you for contacting us.

We do actually recommend storing the lyophilised antibody at -20C for long term storage; this will be stable for 2 years.

The antibody should be stored lyophilised at -20C till use and then reconstituted...

Read More

Thank you for your reply.

Below please find the protocol information you requested:

All solutions should be at ambient temperature prior to use.
PBS: Dilute 10xPBS to 1xPBS, pH 7.20 in sterile water.
Wash ...

Read More

Thank you for contacting Abcam regarding the sELISA protocol using ab83132 and ab9570.

I have confirmed with the laboratory that a streptavidn-peroxidase was used for detection. The catalog number for this product is ab64269.

I ho...

Read More