製品の概要

  • 製品名Anti-VEGFA antibody
    VEGFA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VEGFA
  • 特異性This antibody reacts with the 165, 189 and 121 amino acid splice variants of VEGFA of human, to a lesser extent, mouse and rat.
  • アプリケーション適用あり: IHC-FrFl, IHC-P, IHC-FoFr, IP, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human VEGFA (N terminal).

  • ポジティブ・コントロール
    • Tumor cells in hemangiosarcomas IF/ICC: HepG2 cell line

アプリケーション

Our Abpromise guarantee covers the use of ab39250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FrFl Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr 1/100.
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
  • 組織特異性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
  • 関連疾患Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 配列類似性Belongs to the PDGF/VEGF growth factor family.
  • 細胞内局在Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC70609 antibody
    • MVCD1 antibody
    • Vascular endothelial growth factor A antibody
    • vascular endothelial growth factor antibody
    • Vascular permeability factor antibody
    • VEGF A antibody
    • Vegf antibody
    • VEGF-A antibody
    • VEGF120 antibody
    • Vegfa antibody
    • VEGFA_HUMAN antibody
    • VPF antibody
    see all

Anti-VEGFA antibody 画像

  • ICC/IF image of ab39250 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39250, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Human angiosarcoma stained with ab39250.
  • ab39250 staining VEGF in mouse brain tissue by Immunohistochemistry (Free floating sections). A heat mediated antigen retrieval step (100°C for 20 minutes) was performed using 10mM sodium citrate buffer, 0.05% Tween 20 pH 6.0. Samples were then incubated with the primary antibody at a 1/100 dilution for 72 hours at 4°C. An Alexa Fluor®488-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/500 dilution.ab39250 staining VEGF in mouse brain tissue by Immunohistochemistry (Free floating sections). A heat mediated antigen retrieval step (100°C for 20 minutes) was performed using 10mM sodium citrate buffer, 0.05% Tween 20 pH 6.0. Samples were then incubated with the primary antibody at a 1/100 dilution for 72 hours at 4°C. An Alexa Fluor®488-conjugated goat anti-rabbit polyclonal was used as secondary antibody at a 1/500 dilution.

    See Abreview

Anti-VEGFA antibody (ab39250) 使用論文

This product has been referenced in:
  • Spina A  et al. NZ-GMP Approved Serum Improve hDPSC Osteogenic Commitment and Increase Angiogenic Factor Expression. Front Physiol 7:354 (2016). ICC/IF ; Human . Read more (PubMed: 27594842) »
  • Kim GI  et al. Electrochemical detection of vascular endothelial growth factors (VEGFs) using VEGF antibody fragments modified Au NPs/ITO electrode. Biosens Bioelectron 25:1717-22 (2010). Read more (PubMed: 20047825) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us and your interst in our products.
As discussed over the phone, the Anti-VEGF antibody (ab39250) has not as yet been used in western blotting as you are hoping to do. This does not mean that it would not work, simply tha...

Read More

ab39250 is against VEGFA (VEGF). We do not have any experimental data regarding the cross-reactivity of E2614 with VEGFB, VEGFC, or VEGFD. The percentage homology between the immunogen and the other variants of VEGF are as follows: VEGFB (P497...

Read More
Application Immunohistochemistry free floating
Sample Mouse Tissue sections (brain)
Specification brain
Username

Ms. Christina Whiteus

Verified customer

投稿 Oct 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"