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Recombinant fragment corresponding to Human VEGF Receptor 3. Recombinant human soluble VEGFR3/FLT4 protein (110 kDa) (D1-D7).
Database link: P35916
Our Abpromise guarantee covers the use of ab10284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 1 - 5 µg/ml. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||Use a concentration of 1 - 5 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 164 kDa.|
|IP||Use a concentration of 1 - 5 µg/ml. PubMed: 19366703|
Immunohistochemistry (Frozen sections) analysis of human spleen tissue labelling VEGF Receptor 3 with ab10284.
Flow Cytometry analysis of human dermal lymphatic endothelial cells (HDLEC) labelling VEGF Receptor 3 with ab10284.
Immunocytochemistry/Immunofluorescence analysis of human VEGF Receptor 3 transfected MG63 cells labelling VEGF Receptor 3 (red) with ab10284 (D). A and C are negative controls with secondary antibody only. B uses a monoclonal antibody specific for VEGF Receptor 3.
Serum-starved porcine aortic endothelial cells were incubated with recombinant VEGF proteins, and the phosphorylation status of VEGFRs or Akt was analyzed from cell lysates. The effect of VEGF-D?N?C and C25L on the phosphorylation of VEGFR-3 in PAE-Flt4 cells at 5, 15, and 30 minute time points. Cell lysates were immunoprecipitated using ab10284 and analyzed by immunoblotting with an anti phosphotyrosine antibody. Equal loading was confirmed using ab10284.
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