The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
Use a concentration of 1 - 5 µg/ml.
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 164 kDa.
Use a concentration of 1 - 5 µg/ml. PubMed: 19366703
機能Receptor for VEGFC. Has a tyrosine-protein kinase activity.
組織特異性Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain.
関連疾患Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment. Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue.
配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. Contains 7 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.
Immunocytochemistry/Immunofluorescence analysis of human VEGF Receptor 3 transfected MG63 cells labelling VEGF Receptor 3 (red) with ab10284 (D). A and C are negative controls with secondary antibody only. B uses a monoclonal antibody specific for VEGF Receptor 3.
Immunoprecipitation - VEGF Receptor 3 antibody (ab10284)Image from Toivanen PI et al, J Biol Chem. 2009 Jun 5;284(23):16037-48. Epub 2009 Apr 14, Fig 7. DOI 10.1074/jbc.M109.001123
Serum-starved porcine aortic endothelial cells were incubated with recombinant VEGF proteins, and the phosphorylation status of VEGFRs or Akt was analyzed from cell lysates. The effect of VEGF-D?N?C and C25L on the phosphorylation of VEGFR-3 in PAE-Flt4 cells at 5, 15, and 30 minute time points. Cell lysates were immunoprecipitated using ab10284 and analyzed by immunoblotting with an anti phosphotyrosine antibody. Equal loading was confirmed using ab10284.
Anti-VEGF Receptor 3 antibody (ab10284) 使用論文
This product has been referenced in:
Toivanen PI et al. Novel vascular endothelial growth factor D variants with increased biological activity. J Biol Chem284:16037-48 (2009).
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