製品の概要

  • 製品名
    Anti-VEGF Receptor 2 antibody [EIC]
    VEGF Receptor 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [EIC] to VEGF Receptor 2
  • 特異性
    The monoclonal antibody will detect native human VEGFR-2 / KDR in ELISA experiments and on the surface of different human cell types. It recognizes the transmembrane KDR e.g. in HUVECs which normally is fully glycosylated (200kDa form).
  • アプリケーション
    適用あり: ICC/IF, IHC-P, ELISA, IHC-Fr, IHC - Wholemount, Flow Cytmore details
    適用なし: WB
  • 種交差性
    交差種: Rat, Sheep, Human
  • 免疫原

    Recombinant fragment corresponding to Human VEGF Receptor 2 aa 20-757. Recombinant human soluble VEGF Receptor 2.
    Database link: P35968

  • ポジティブ・コントロール
    • HUVEC cells. IHC-P: FFPE human placenta.

アプリケーション

Our Abpromise guarantee covers the use of ab9530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 16763549
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use a concentration of 1 - 5 µg/ml.
IHC-Fr Use a concentration of 1 µg/ml.
IHC - Wholemount Use at an assay dependent concentration.
Flow Cyt Use a concentration of 2 - 10 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • 関連疾患
      Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
    • 配列類似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD309 antibody
      • CD309 antigen antibody
      • EC 2.7.10.1 antibody
      • Fetal liver kinase 1 antibody
      • FLK-1 antibody
      • FLK1 antibody
      • FLK1, mouse, homolog of antibody
      • Kdr antibody
      • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
      • Kinase insert domain receptor antibody
      • KRD1 antibody
      • Ly73 antibody
      • Protein tyrosine kinase receptor FLK1 antibody
      • Protein-tyrosine kinase receptor flk-1 antibody
      • soluble VEGFR2 antibody
      • Tyrosine kinase growth factor receptor antibody
      • Vascular endothelial growth factor receptor 2 antibody
      • VEGFR 2 antibody
      • VEGFR antibody
      • VEGFR-2 antibody
      • VEGFR2 antibody
      • VGFR2_HUMAN antibody
      see all

    画像

    • Detection of VEGFR-2 extracellular domain of human umbilical vein endothelial cells (HUVEC) using monoclonal antibody VEGFR-2  (bold) versus control conditions.

    • IHC image of VEGF Receptor 2 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9530, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Immunocytochemical analysis of human aortic endothelial cells, labelling VEGF Receptor 2 with ab9530 (green). Cells cultured until 70% confluent and fixed with 4% paraformaldehyde for 15 minutes at room temperature. They were then incubated with ab9530, diluted in PBS containing 5% normal goat serum and 0.01% Triton X-100 at 4°C overnight. DAPI used to stain nuclei. 

    • Immunostaining of myocardium section. The arrowheads indicate VEGFR-2 positive endothelial cells from myocardial capillaries.

    • ab9530 staining Human aortic endothelial cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/2000 in PBS + 0.1% Triton-X 100 + 5% Goat serum) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Green - VEGF Receptor 2, Red - alpha tubulin, Blue - nuclear marker (DAPI).

      See Abreview

    • Wholemount Immunohistochemical analysis of lung tissue from a 6 day postnatal mouse, labelling VEGF Receptor 2 with ab9530. ab9530 was diluted 1/500 in 3% BSA and 0.1% Triton 100 in PBS buffer, and incubated at 4°C for 12 hours. The secondary used was a polyclonal Alexa Fluor 488 Goat Anti-Mouse IgG at 1/1000.

      See Abreview

    • ab9530 staining rat lung cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde and  blocked in 5% normal goat serum for 1 hour at room temperature. Samples were incubated with primary antibody (dilution 1/50) for 14 hours at 4°C; the diution buffer was 5% NGS in 1 X TBS-T (0.1%tween20). A Cy3® -conjugated Goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.

      See Abreview

    参考文献

    This product has been referenced in:
    • Al-Maqtari T  et al. Transcription factor-induced activation of cardiac gene expression in human c-kit+ cardiac progenitor cells. PLoS One 12:e0174242 (2017). Read more (PubMed: 28355297) »
    • Hong X  et al. Transdifferentiated Human Vascular Smooth Muscle Cells are a New Potential Cell Source for Endothelial Regeneration. Sci Rep 7:5590 (2017). Read more (PubMed: 28717251) »

    See all 19 Publications for this product

    レビューと Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37°C
    Sample
    Cow Cell (Bovine Aortic Endothelial Cell)
    Specification
    Bovine Aortic Endothelial Cell
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Formaldehyde
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    投稿 Feb 09 2015

    Application
    Flow Cytometry
    Fixation
    Paraformaldehyde
    Sample
    Human Cell (HUVEC, iPSC)
    Specification
    HUVEC, iPSC
    Permeabilization
    No
    Username

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    Verified customer

    投稿 Jul 15 2014

    Application
    IHC - Wholemount
    Sample
    Mouse Tissue (Postnatal day 6 mouse lung)
    Specification
    Postnatal day 6 mouse lung
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    投稿 Dec 18 2013

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Antigen retrieval step
    None
    Sample
    Human Tissue sections (Human epithelial ovarian carcinoma tissue)
    Specification
    Human epithelial ovarian carcinoma tissue
    Permeabilization
    Yes - 0.2% Triton X-100
    Fixative
    Paraformaldehyde
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    投稿 Dec 18 2013

    Application
    Immunocytochemistry
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 23°C
    Sample
    Mouse Cultured Cells (Mouse endothelial cells)
    Specification
    Mouse endothelial cells
    Permeabilization
    No
    Fixative
    Paraformaldehyde
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    Verified customer

    投稿 Dec 18 2013

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample
    Human Cell (Human aortic endothelial cells)
    Specification
    Human aortic endothelial cells
    Permeabilization
    Yes - 0.1% Triton X-100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Oct 17 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Sheep Cell (Pituitary)
    Specification
    Pituitary
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    Verified customer

    投稿 Feb 25 2013

    Thank you for your reply. I have now returned to the office an hope I can help further with your enquiry.

    I can now confirm that of the antibodies we have directed against VEGF Receptor 2 (that are not raised in rabbit), most likely to react w...

    Read More

    Thank you for your reply. I have now checked through the antibodies we have directed against VEGF Receptor 2 and the likelihood that any of them will be able to cross react with the protein in your sheep samples.

    The two fragments of sequence ...

    Read More

    Thank you for your enquiry and interest.

    In order to be able to find the suitable isotype control, it is important to know the isotype of these antibodies are:

    ab9530 - Mouse IgG1

    ab27619 - Rabbit IgG (not determined furth...

    Read More

    1-10 of 36 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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