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Recombinant fragment corresponding to Human VEGF Receptor 2 aa 20-757. Recombinant human soluble VEGF Receptor 2.
Database link: P35968
Our Abpromise guarantee covers the use of ab9530 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 16763549|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use a concentration of 1 - 5 µg/ml.|
|IHC-Fr||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use a concentration of 2 - 10 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC image of VEGF Receptor 2 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9530, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab9530 staining Human aortic endothelial cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/2000 in PBS + 0.1% Triton-X 100 + 5% Goat serum) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Green - VEGF Receptor 2, Red - alpha tubulin, Blue - nuclear marker (DAPI).
Detection of VEGFR-2 extracellular domain of human umbilical vein endothelial cells (HUVEC) using monoclonal antibody VEGFR-2 (bold) versus control conditions.
Immunostaining of myocardium section. The arrowheads indicate VEGFR-2 positive endothelial cells from myocardial capillaries.