製品の概要

  • 製品名Anti-VEGF Receptor 2 antibody [EIC]
    VEGF Receptor 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [EIC] to VEGF Receptor 2
  • 特異性The monoclonal antibody will detect native human VEGFR-2 / KDR in ELISA experiments and on the surface of different human cell types. It recognizes the transmembrane KDR e.g. in HUVECs which normally is fully glycosylated (200kDa form).
  • アプリケーション適用あり: ICC/IF, IHC-P, ELISA, IHC-Fr, Flow Cytmore details
    適用なし: WB
  • 種交差性
    交差種: Rat, Sheep, Human
  • 免疫原

    Recombinant fragment corresponding to Human VEGF Receptor 2 aa 20-757. Recombinant human soluble VEGF Receptor 2.
    Database link: P35968

  • ポジティブ・コントロール
    • HUVEC cells. IHC-P: FFPE human placenta.

アプリケーション

Our Abpromise guarantee covers the use of ab9530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 16763549
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use a concentration of 1 - 5 µg/ml.
IHC-Fr Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 2 - 10 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • 関連疾患Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.
    • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
      Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CD309 antibody
      • CD309 antigen antibody
      • EC 2.7.10.1 antibody
      • Fetal liver kinase 1 antibody
      • FLK-1 antibody
      • FLK1 antibody
      • FLK1, mouse, homolog of antibody
      • Kdr antibody
      • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
      • Kinase insert domain receptor antibody
      • KRD1 antibody
      • Ly73 antibody
      • Protein tyrosine kinase receptor FLK1 antibody
      • Protein-tyrosine kinase receptor flk-1 antibody
      • soluble VEGFR2 antibody
      • Tyrosine kinase growth factor receptor antibody
      • Vascular endothelial growth factor receptor 2 antibody
      • VEGFR 2 antibody
      • VEGFR antibody
      • VEGFR-2 antibody
      • VEGFR2 antibody
      • VGFR2_HUMAN antibody
      see all

    Anti-VEGF Receptor 2 antibody [EIC] 画像

    • IHC image of VEGF Receptor 2 staining in human placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9530, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • ab9530 staining Human aortic endothelial cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/2000 in PBS + 0.1% Triton-X 100 + 5% Goat serum) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Green - VEGF Receptor 2, Red - alpha tubulin, Blue - nuclear marker (DAPI).

      See Abreview

    • Detection of VEGFR-2 extracellular domain of human umbilical vein endothelial cells (HUVEC) using monoclonal antibody VEGFR-2  (bold) versus control conditions.

    • Immunostaining of myocardium section. The arrowheads indicate VEGFR-2 positive endothelial cells from myocardial capillaries.

    • ab9530 staining rat lung cells by ICC/IF (Immunocytochemistry/immunoflurescence). Cells were fixed with paraformaldehyde and  blocked in 5% normal goat serum for 1 hour at room temperature. Samples were incubated with primary antibody (dilution 1/50) for 14 hours at 4°C; the diution buffer was 5% NGS in 1 X TBS-T (0.1%tween20). A Cy3® -conjugated Goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.

      See Abreview

    Anti-VEGF Receptor 2 antibody [EIC] (ab9530) 使用論文

    This product has been referenced in:
    • Sun D  et al. Molecular imaging reveals elevated VEGFR-2 expression in retinal capillaries in diabetes: a novel biomarker for early diagnosis. FASEB J 28:3942-51 (2014). IHC ; Rat . Read more (PubMed: 24903276) »
    • Tengroth L  et al. Deprived TLR9 expression in apparently healthy nasal mucosa might trigger polyp-growth in chronic rhinosinusitis patients. PLoS One 9:e105618 (2014). Flow Cyt ; Human . Read more (PubMed: 25133733) »

    See all 14 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 37°C
    Sample Cow Cell (Bovine Aortic Endothelial Cell)
    Specification Bovine Aortic Endothelial Cell
    Permeabilization Yes - 0.1% Triton X-100
    Fixative Formaldehyde
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    投稿 Feb 09 2015

    Application Flow Cytometry
    Fixation Paraformaldehyde
    Sample Human Cell (HUVEC, iPSC)
    Specification HUVEC, iPSC
    Permeabilization No
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    投稿 Jul 15 2014

    Application IHC - Wholemount
    Sample Mouse Tissue (Postnatal day 6 mouse lung)
    Specification Postnatal day 6 mouse lung
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    投稿 Dec 18 2013

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Antigen retrieval step None
    Sample Human Tissue sections (Human epithelial ovarian carcinoma tissue)
    Specification Human epithelial ovarian carcinoma tissue
    Permeabilization Yes - 0.2% Triton X-100
    Fixative Paraformaldehyde
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    投稿 Dec 18 2013

    Application Immunocytochemistry
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 3% · Temperature: 23°C
    Sample Mouse Cultured Cells (Mouse endothelial cells)
    Specification Mouse endothelial cells
    Permeabilization No
    Fixative Paraformaldehyde
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    投稿 Dec 18 2013

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
    Sample Human Cell (Human aortic endothelial cells)
    Specification Human aortic endothelial cells
    Permeabilization Yes - 0.1% Triton X-100
    Fixative Paraformaldehyde
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    投稿 Oct 17 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Sheep Cell (Pituitary)
    Specification Pituitary
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    投稿 Feb 25 2013

    Thank you for your reply. I have now returned to the office an hope I can help further with your enquiry.

    I can now confirm that of the antibodies we have directed against VEGF Receptor 2 (that are not raised in rabbit), most likely to react w...

    Read More

    Thank you for your reply. I have now checked through the antibodies we have directed against VEGF Receptor 2 and the likelihood that any of them will be able to cross react with the protein in your sheep samples.

    The two fragments of sequence ...

    Read More

    Thank you for your enquiry and interest.

    In order to be able to find the suitable isotype control, it is important to know the isotype of these antibodies are:

    ab9530 - Mouse IgG1

    ab27619 - Rabbit IgG (not determined furth...

    Read More

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"