製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 151 kDa.
IHC-P 1/250.
ICC/IF 1/250.
IP 1/100.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-Fr 1/250.

ターゲット情報

  • 機能Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
  • 組織特異性Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 2.7.10.1 antibody
    • FLT 1 antibody
    • FLT antibody
    • Flt-1 antibody
    • FLT1 antibody
    • Fms like tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody
    • Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody
    • Fms-like tyrosine kinase 1 antibody
    • FRT antibody
    • Soluble VEGF receptor 1 14 antibody
    • Soluble VEGFR1 variant 2 antibody
    • Soluble VEGFR1 variant 21 antibody
    • Tyrosine protein kinase FRT antibody
    • Tyrosine protein kinase receptor FLT antibody
    • Tyrosine-protein kinase FRT antibody
    • Tyrosine-protein kinase receptor FLT antibody
    • Vascular endothelial growth factor receptor 1 antibody
    • Vascular endothelial growth factor vascular permeability factor receptor antibody
    • Vascular permeability factor receptor 1 antibody
    • Vascular permeability factor receptor antibody
    • VEGFR 1 antibody
    • VEGFR-1 antibody
    • VEGFR1 antibody
    • VGFR1_HUMAN antibody
    see all

Anti-VEGF Receptor 1 antibody [Y103] 画像

  • Immunohistochemistry (PFA perfusion fixed frozen sections) analysis of mouse brain tissue section (15 days old wild-type mouse embryonic brain, 16 micron) labeling VEGF Receptor 1 with ab32152 at 1/300 dilution. Tissue was fixed with formaldehyde and permeabilized with Triton X100. Heat mediated antigen retrieval was performed using 10mM citrate buffer, pH 6. A polyclonal donkey anti-Rabbit IgG (H+L) (Alexa Fluor® 488) secondary antibody was used at 1/500 dilution. 

    See Abreview

  • Immunocytochemistry/ Immunofluorescence analysis of mouse cardiac cells labeling VEGF Receptor 1 with ab32152. An Alexa Fluor-conjugated secondary antibody was used. Blue: DAPI counterstaining, Green : GFP, Red: VEGF Receptor 1.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human abdominal aortic aneurysm (AAA) wall tissue sections labeling VEGF Receptor 1 with ab32152 at 1/100 dilution.

    Resected aortic tissues were immersed in 10% neutral buffered formalin for at least 24 h for immunohistochemical staining. Tissue sample was embedded in paraffin; 4 µm sections were cut and mounted onto MAS-coated slides. The sections were deparaffinized, dehydrated, and boiled in a pressure cooker in 0.01 M citric acid buffer (pH 6.0) for 20 min. The sections were washed with phosphate-buffered saline and incubated with 3% H2O2 in absolute methanol for 5 min to inhibit any endogenous peroxidase activity. Sections were preincubated with 3% normal goat serum for 20 min to minimize nonspecific binding to VEGF Receptor 1, and incubated with ab32152 at 4°C overnight in a moist chamber. The section was washed with phosphate-buffered saline and then incubated with the appropriate secondary antibody for 30 min at room temperature. Staining was visualized with Vector DAB, and tissue section was then counterstained with hematoxylin.

  • Overlay histogram showing A431 cells stained with ab32152 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32152, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used a goat anti-rabbit DyLight® 488 goat (IgG, H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Anti-VEGF Receptor 1 antibody [Y103] (ab32152) at 1/10000 dilution + mouse brain tissue

    Predicted band size : 151 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
  • Flow Cytometry analysis of A7r5 (left) and A10 (middle) and MVSC (right) cells labeling VEGF Receptor 1 with ab32152. Open curves represent negative control samples; red filled curves represent samples stained with ab32152.

    Cells were cultured until confluency in T75 flasks and then trypsinised in 2x trypsin/0.53 mM EDTA at 37°C. Cells were resuspended in media, counted, and then fixed for 20 mins at 4°C. Cells were then washed by centrifugation at 500 g for 3 mins. Following washing, cells were resuspended in solution containing 1 μg of Anti-VEGF Receptor 1 antibody [Y103] (ab32152) and incubated at 4°C for 30 mins. Following washing, cells were resuspended in solution containing 1 μg of appropriate enzyme-linked secondary antibody and incubated at 4°C for 30 mins. Cells were then washed in solution and resuspended in a final volume of 500 μL of solution.

Anti-VEGF Receptor 1 antibody [Y103] (ab32152) 使用論文

This product has been referenced in:
  • Zhou W  et al. Fibroblast growth factor receptor 1 promotes MG63 cell proliferation and is associated with increased expression of cyclin-dependent kinase 1 in osteosarcoma. Mol Med Rep 13:713-9 (2016). WB ; Human . Read more (PubMed: 26648125) »
  • Liu X  et al. Impaired VEGF Signaling in Lungs with Hypoplastic Esophageal Atresia and Effects on Branching Morphogenesis. Cell Physiol Biochem 39:385-94 (2016). IHC ; Rat . Read more (PubMed: 27372649) »

See all 69 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
Sample Mouse Tissue sections (15 days old WT embryonic brain, 16 micron)
Specification 15 days old WT embryonic brain, 16 micron
Permeabilization Yes - Triton X100
Fixative Formaldehyde
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投稿 Mar 06 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (liver, Hep G2)
Specification liver, Hep G2
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jan 06 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver, hepatocytes)
Specification liver, hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Jan 02 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8% SDS-PAGE)
Sample Human Cell lysate - whole cell (HUVECs)
Specification HUVECs
Treatment 10ng/ml IL-1 beta
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 May 30 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Tissue lysate - whole (Placenta)
Specification Placenta
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
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投稿 Jan 30 2014

ab32152 was tested with a permeabilization step but the immunogen sequence and SwissProt suggests an extracellular location. Whilst collating testing conditions, permeabilization was assigned based on “Secreted and Cell membrane” from the c...

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After looking into our data for ab32152, I found the following abReview, which was submitted by another researcher using ab32152 in immunocytochemistry. According to the researcher's protocol (link below) permeabilization was not necessary, which ...

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Thank you for your email.

The concentration of the ab is 15ug/ml which means 0.015 ug/ul.

The 100ul of antibody will concentration 1.5ug per 100ul.

This antibody is rabbit monoclonal antibody which is different in specifici...

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Thank you for contacting us.

The concentration of lot is GR106697 is 15 ug/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Vielen Dank für Ihre Anfrage.

Bei dem Anti-VEGF Receptor 1 antibody [Y103] (ab32152) handelt es sich um einen Antikoerper in "Tissue culture supernatant". Dieser wurde aufgereinigt, so dass nur noch Proteine mit der IgG Fraktion enthalten sind...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"