Anti-VEGF Receptor 1 抗体 [Flt-1/EWC] (ab9540)

製品の概要

  • 製品名Anti-VEGF Receptor 1 antibody [Flt-1/EWC]
    VEGF Receptor 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Flt-1/EWC] to VEGF Receptor 1
  • 特異性Detects native and denaturated VEGFR-1/ Flt-1
  • アプリケーション適用あり: ELISA, WB, ICC/IF, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Dog, Human
  • 免疫原

    Recombinant human soluble extracellular Flt-1 Ig-like loop 1 to 5 (sFlt-1(D5)).

  • ポジティブ・コントロール
    • recombinant soluble VEGFR 1, soluble human VEGFR-1 from human follicular fluids (see Neulen et al), mouse placenta tissue
  • 特記事項

    VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity. Because sFLT1 has a higher affinity for VEGF than does FLK1, it may function as an inhibitor of VEGF response.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 2 - 5 µg/ml.
ICC/IF 1/50.
IHC-P Use at an assay dependent concentration. PubMed: 17823277
Flow Cyt Use at an assay dependent concentration. PubMed: 25223735

ターゲット情報

  • 機能Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
  • 組織特異性Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 2.7.10.1 antibody
    • FLT 1 antibody
    • FLT antibody
    • Flt-1 antibody
    • FLT1 antibody
    • Fms like tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody
    • Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody
    • Fms-like tyrosine kinase 1 antibody
    • FRT antibody
    • Soluble VEGF receptor 1 14 antibody
    • Soluble VEGFR1 variant 2 antibody
    • Soluble VEGFR1 variant 21 antibody
    • Tyrosine protein kinase FRT antibody
    • Tyrosine protein kinase receptor FLT antibody
    • Tyrosine-protein kinase FRT antibody
    • Tyrosine-protein kinase receptor FLT antibody
    • Vascular endothelial growth factor receptor 1 antibody
    • Vascular endothelial growth factor vascular permeability factor receptor antibody
    • Vascular permeability factor receptor 1 antibody
    • Vascular permeability factor receptor antibody
    • VEGFR 1 antibody
    • VEGFR-1 antibody
    • VEGFR1 antibody
    • VGFR1_HUMAN antibody
    see all

Anti-VEGF Receptor 1 antibody [Flt-1/EWC] 画像

  • Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of Human Placenta, Placental Bed and Decidua Parietalis. VEGF Receptor 1 (geen) was labelled with ab9540 and VEGF Receptor 2 (red) was labelled with ab2349. Yellow - where both VEGF Receptor 1 and VEGF Receptor 2 are expressed. DAPI was used for nuclei staining (blue).

Anti-VEGF Receptor 1 antibody [Flt-1/EWC] (ab9540) 使用論文

This product has been referenced in:
  • Ostyn P  et al. Transient TNF regulates the self-renewing capacity of stem-like label-retaining cells in sphere and skin equivalent models of melanoma. Cell Commun Signal 12:52 (2014). Flow Cyt ; Human . Read more (PubMed: 25223735) »
  • Suspitsin EN  et al. Evidence for angiogenesis-independent contribution of VEGFR1 (FLT1) in gastric cancer recurrence. Med Oncol 30:644 (2013). Read more (PubMed: 23801279) »

See all 13 Publications for this product

Product Wall

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by pass...

Read More

Thank you for your message. I can confirm that the ab9540 VEGF Receptor 1 antibody [Flt-1/EWC] was generated using the recombinant soluble FLT-1 (domain 1-5) for immunization of mouse. Therefore it detects the soluble as well as the transmembran...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (endothelial cells)
Specification endothelial cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton/0.1% NP-40
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Oct 17 2008

I'm sorry to hear you are having a problem with ab9540. I checked the enquiries regarding this antibody and I didn't see any other complaints of differences between lots since November of last year. Since you have used this antibody successfully ...

Read More

Thank you for your enquiry. The binding epitope is located in ecto domain D1 or D2. It is not further characterized. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Thank you for your enquiry. We do not know if the antibody recognizes the receptor when bound with its ligand. We can confirm that the antibody recognizes the receptor unbound as the antibody will detect denaturated human VEGFR-1/ Flt-1 in ELISA experi...

Read More

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab9540. It is strange that you are not seeing any signal at all with the HUVEC sample and you are with the human placenta trophoblast samples. You may need to lo...

Read More

Thank you for submitting for your complaint and for providing more details of your protocol. Looking at our database, we can see that we have sold several vials of batch: 68526 without any problem. It may well be that the vial you received has just...

Read More

Thank you for your enquiry and your patience. We would like to confirm that this antibody recognizes both forms. IP is a normal standard protocol: 1-2µg antibody, 30µl anti-mouse IgG activated Sepharose beads (Sigma), incubation for 3-4 hours o...

Read More

To our knowledge this antibody reacts with mouse 3T3 cells transfected with the mouse flt-1 cDNA in Western blotting. This was shown by Dr. Georg Breier (MPI Bad Nauheim, Germany). We are not sure whether the endogenous expression of Flt-1 or sFlt-1 i...

Read More

1-10 of 12 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"