製品の概要
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製品名Anti-VEGF 165A antibody
VEGF 165A 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to VEGF 165A
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特異性ab106580 will recognize both native and recombinant Human VEGF 165A in cell supernatants and certain body fluids. The immunogen is full length recombinant human VEGF-165 protein, but this has high homology with multiple VEGF isoforms, therefore it should recognise these other isoforms too.
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アプリケーション適用あり: WB, ELISAmore details
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種交差性交差種: Human
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免疫原
Recombinant full length protein corresponding to Human VEGF 165A.
Database link: P15692-4 -
ポジティブ･コントロール
- Recombinant Human VEGF 165A
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.20
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading... -
精製度Ion Exchange Chromatography
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特記事項（精製）ab106580 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated. This purified antibody has been heated to 56°C for 30 minutes. The endotoxin content is estimated to be <10 pg/µl by the LAL method.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab106580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). | |
| ELISA | 1/10000. |
ターゲット情報
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機能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
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組織特異性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
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関連疾患Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
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配列類似性Belongs to the PDGF/VEGF growth factor family.
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細胞内局在Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
- Information by UniProt
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参照データベース
- Entrez Gene: 7422 Human
- Omim: 192240 Human
- SwissProt: P15692 Human
- Unigene: 73793 Human
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別名
- glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
- MVCD1 antibody
see all
Anti-VEGF 165A antibody 画像
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Western blot - Anti-VEGF 165A antibody (ab106580)Anti-VEGF 165A antibody (ab106580) at 1/1000 dilution + Recombinant Human VEGF 165A
Secondary
Peroxidase conjugated anti-Rabbit IgG at 1/40000 dilution
Predicted band size : 22 kDa
プロトコール
Anti-VEGF 165A antibody (ab106580) 使用論文
ab106580 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"