Anti-VEGF 165A 抗体 (ab106580)
Key features and details
- Rabbit polyclonal to VEGF 165A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-VEGF 165A antibody
VEGF 165A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VEGF 165A -
由来種
Rabbit -
特異性
ab106580 will recognize both native and recombinant Human VEGF 165A in cell supernatants and certain body fluids. The immunogen is full length recombinant human VEGF-165 protein, but this has high homology with multiple VEGF isoforms, therefore it should recognise these other isoforms too. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human VEGF 165A.
Database link: P15692-4 -
ポジティブ・コントロール
- WB: Recombinant human VEGF 165A.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
特記事項(精製)
ab106580 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated. This purified antibody has been heated to 56°C for 30 minutes. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106580の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
ターゲット情報
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機能
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
組織特異性
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
関連疾患
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
配列類似性
Belongs to the PDGF/VEGF growth factor family. -
細胞内局在
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
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参照データベース
- Entrez Gene: 7422 Human
- Omim: 192240 Human
- SwissProt: P15692 Human
- Unigene: 73793 Human
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別名
- glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
- MVCD1 antibody
see all
画像
データシートおよび資料
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Datasheet download
参考文献 (2)
ab106580 は 2 報の論文で使用されています。
- Chen H et al. VEGF165 gene-modified human umbilical cord blood mesenchymal stem cells protect against acute liver failure in rats. J Gene Med 23:e3369 (2021). PubMed: 34057770
- Yan Y et al. Modeling of diseases of retinal ischemia in vitro: possible participation of autocrine vascular endothelial growth factor signaling. Ophthalmic Res 49:90-9 (2013). PubMed: 23257772