Anti-VEGF 164 抗体 (ab53465)
Key features and details
- Rabbit polyclonal to VEGF 164
- Suitable for: IHC-P, RIA, ELISA, WB
- Reacts with: Rat, Cow, Human, Pig
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-VEGF 164 antibody -
製品の詳細
Rabbit polyclonal to VEGF 164 -
由来種
Rabbit -
特異性
ab53465 reacts with all human VEGF isoforms (121, 165, 189, and 206). No cross reaction is observed with PDGF, FGF-1, FGF-2, and TGFa. -
アプリケーション
適用あり: IHC-P, RIA, ELISA, WBmore details -
種交差性
交差種: Rat, Cow, Human, Pig -
免疫原
Recombinant bovine VEGF 164
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53465の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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RIA |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration. PubMed: 21441982
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. PubMed: 21441982 |
WB
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa. |
ターゲット情報
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機能
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
組織特異性
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland. -
関連疾患
Microvascular complications of diabetes 1 -
配列類似性
Belongs to the PDGF/VEGF growth factor family. -
細胞内局在
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
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参照データベース
- Entrez Gene: 7422 Human
- Entrez Gene: 83785 Rat
- Omim: 192240 Human
- SwissProt: P15691 Cow
- SwissProt: P15692 Human
- SwissProt: P49151 Pig
- SwissProt: P16612 Rat
- Unigene: 73793 Human
see all -
別名
- Vascular endothelial growth factor A antibody
- Vascular permeability factor antibody
- VEGF antibody
see all
画像
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Anti-VEGF 164 antibody (ab53465) at 1/500 dilution + Human thymus tissue lysate - total protein (ab30146) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 27 kDa
Observed band size: 27 kDa
Additional bands at: 70 kDa (possible dimer), 70 kDa (possible glycosylated form)
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (54)
ab53465 は 54 報の論文で使用されています。
- Wang Y et al. Loading of erythropoietin on biphasic calcium phosphate bioceramics promotes osteogenesis and angiogenesis by regulating EphB4/EphrinB2 molecules. J Mater Sci Mater Med 33:19 (2022). PubMed: 35072831
- Sui T et al. Gambogic amide inhibits angiogenesis by suppressing VEGF/VEGFR2 in endothelial cells in a TrkA-independent manner. Pharm Biol 59:1566-1575 (2021). PubMed: 34767490
- Zhu L & Liu Z Serum from patients with hypertension promotes endothelial dysfunction to induce trophoblast invasion through the miR-27b-3p/ATPase plasma membrane Ca2+ transporting 1 axis. Mol Med Rep 23:N/A (2021). PubMed: 33760199
- Zou W et al. ASK1/p38-mediated NLRP3 inflammasome signaling pathway contributes to aberrant retinal angiogenesis in diabetic retinopathy. Int J Mol Med 47:732-740 (2021). PubMed: 33416127
- Liang C et al. Downregulation of astrocyte elevated gene-1 expression inhibits the development of vasculogenic mimicry in gliomas. Exp Ther Med 21:22 (2021). PubMed: 33235631