Anti-VE Cadherin (phospho Y685) 抗体 (ab119785)

製品の概要

  • 製品名Anti-VE Cadherin (phospho Y685) antibody
    VE Cadherin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VE Cadherin (phospho Y685)
  • 特異性ab119785 detects a 140 kDa band corresponding to VE cadherin in western blots of Human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phospho-peptide (Y685), conjugated to carrier protein, corresponding to amino acids surrounding tyrosine 685 in Human VE Cadherin.

  • ポジティブ・コントロール
    • Human umbilical vein endothelial cells stimulated with pervanadate.
  • 特記事項Do not aliquot.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 88 kDa).

ターゲット情報

  • 機能Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton.
  • 組織特異性Endothelial tissues and brain.
  • 配列類似性Contains 5 cadherin domains.
  • 翻訳後修飾Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB.
  • 細胞内局在Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 7B 4 antibody
    • 7B4 antibody
    • 7B4 antigen antibody
    • CADH5_HUMAN antibody
    • Cadherin 5 antibody
    • Cadherin 5 type 2 antibody
    • Cadherin 5, type 2 (vascular endothelium) antibody
    • Cadherin 5, type 2, VE cadherin (vascular epithelium) antibody
    • cadherin, vascular endothelial antibody
    • cadherin, vascular endothelial, 1 antibody
    • Cadherin-5 antibody
    • Cadherin5 antibody
    • CD 144 antibody
    • CD144 antibody
    • CD144 antigen antibody
    • CDH 5 antibody
    • CDH5 antibody
    • CDH5 protein antibody
    • Endothelial specific cadherin antibody
    • FLJ17376 antibody
    • OTTHUMP00000174777 antibody
    • Vascular endothelial cadherin antibody
    • Vascular epithelium cadherin antibody
    • VE Cad antibody
    • VE-cadherin antibody
    • VEC antibody
    see all

Anti-VE Cadherin (phospho Y685) antibody 画像

  • All lanes : Anti-VE Cadherin (phospho Y685) antibody (ab119785) at 1/1000 dilution

    Lane 1 : Human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min.
    Lane 2 : Human umbilical vein endothelial cells stimulated with pervanadate (1 mM) for 30 min then treated with alkaline phosphatase


    Predicted band size : 88 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)

Anti-VE Cadherin (phospho Y685) antibody (ab119785) 使用論文

ab119785 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (lung)
Gel Running Conditions Reduced Denaturing (6%)
Loading amount 30 µg
Specification lung
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Sep 09 2016

Thank you for your inquiry.

I am happy to confirm that both antibodies are tested and guaranteed for human sample in WB.

I would like to assure you that both antibodies are suitable for your purposes.

They are antibodies g...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"