製品の概要

  • 製品名Anti-VDAC2 antibody
    VDAC2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VDAC2
  • 特異性This antibody also reconginizes VDAC3 with which its sequence is 76% homologous.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Cow, Cat, Dog, Human, Chimpanzee, Zebrafish
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Turkey, Pig
  • 免疫原

    Synthetic peptide:

    KKSGKIKSSYKRE

    , corresponding to internal sequence amino acids 120-132 of Human VDAC2

  • ポジティブ・コントロール
    • Human brain cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 22114330
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

Anti-VDAC2 antibody 画像

  • All lanes : Anti-VDAC2 antibody (ab47104) at 2 µg/ml

    Lane 1 : Human brain cell lysate with no immunizing peptide
    Lane 2 : Human brain cell lysate with immunizing peptide


    Predicted band size : 32 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab47104 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab47104, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-VDAC2 antibody (ab47104) at 2 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Human heart mitochondrial extract at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

Anti-VDAC2 antibody (ab47104) 使用論文

This product has been referenced in:
  • Das T  et al. Potential role of Hsp25 in calcium-modulated cardiomyocytes. Proteomics 12:411-20 (2012). WB ; Human . Read more (PubMed: 22140065) »
  • Li Z  et al. Critical role for voltage-dependent anion channel 2 in infectious bursal disease virus-induced apoptosis in host cells via interaction with VP5. J Virol 86:1328-38 (2012). ICC/IF ; Human . Read more (PubMed: 22114330) »

See all 7 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein A
Sample Mouse Tissue lysate - whole (Brainstem, control mice)
Specification Brainstem, control mice
Total protein in input 500 µg
Username

Dr. Norma Frizzell

Verified customer

投稿 Jun 04 2013

Thank you so much.

Please let me know if there is anything else I can help you with.

Thank you for contacting us.

We have the following antibodies for VDAC2 and VDAC3 that have been tested in WB and with human samples:

VDAC2: ab47104, ab37985, ab118872, ab100956, ab77160, ab117587, ab126120

VDAC3: ab3745...

Read More

Thank you for your enquiry. The recognition of VDAC3 is because on the homology betweeen VDAC2 and VDAC3 (peptide immunogen sequence listed on datashee). Thus, a polyclonal antibody made against the particular VDAC sequence would be expected to reco...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"