製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to VDAC2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human VDAC2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Brain tissue lysate as well as both HeLa and HepG2 whole cell lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab100956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).

ターゲット情報

  • 機能
    Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
  • 組織特異性
    Expressed in all tissues examined.
  • 配列類似性
    Belongs to the eukaryotic mitochondrial porin family.
  • ドメイン
    Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.
  • 細胞内局在
    Mitochondrion outer membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • B-36 vdac antibody
    • FLJ23841 antibody
    • hVDAC2 antibody
    • OTTHUMP00000019878 antibody
    • OTTHUMP00000019879 antibody
    • OTTHUMP00000019880 antibody
    • Outer mitochondrial membrane protein porin 2 antibody
    • POR antibody
    • Porin-2 antibody
    • VDAC 2 antibody
    • VDAC-2 antibody
    • VDAC2 antibody
    • VDAC2_HUMAN antibody
    • Vdac6 antibody
    • Voltage dependent anion channel 2 antibody
    • Voltage dependent anion selective channel protein 2 antibody
    • Voltage-dependent anion-selective channel protein 2 antibody
    see all

画像

  • All lanes : Anti-VDAC2 antibody (ab100956) at 1 µg/ml (Milk Blocked - 3%)

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 16 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

プロトコール

参考文献

ab100956 has not yet been referenced specifically in any publications.

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