製品の概要

  • 製品名Anti-VDAC1/Porin antibody
    VDAC1/Porin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to VDAC1/Porin
  • アプリケーション適用あり: WB, Flow Cyt, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Cynomolgus Monkey, Rhesus monkey, Opossum
  • 免疫原

    Recombinant fragment corresponding to Human VDAC1/Porin aa 1-100.
    Sequence:

    MAVPPTYADLGKSARDVFTKGYGFGLIKLDLKTKSENGLEFTSSGSANTE TTKVTGSLETKYRWTEYGLTFTEKWNTDNTLGTEITVEDQLARGLKLTFD

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 31 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
  • 組織特異性Heart, liver and skeletal muscle.
  • 配列類似性Belongs to the eukaryotic mitochondrial porin family.
  • ドメインConsists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity.
  • 細胞内局在Mitochondrion outer membrane. Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • N2441 antibody
    • OMP2 antibody
    • POR1 antibody
    • hVDAC1 antibody
    • MGC111064 antibody
    • Mitochondrial Porin antibody
    • Outer mitochondrial membrane protein porin 1 antibody
    • Plasmalemmal porin antibody
    • Porin 31HL antibody
    • Porin 31HM antibody
    • VDAC antibody
    • VDAC-1 antibody
    • Vdac1 antibody
    • VDAC1_HUMAN antibody
    • Voltage dependent anion channel 1 antibody
    • Voltage dependent anion selective channel protein 1 antibody
    • Voltage-dependent anion-selective channel protein 1 antibody
    • YNL055C antibody
    • YNL2441C antibody
    see all

Anti-VDAC1/Porin antibody 画像



  • Predicted band size : 31 kDa
    Western blot against tagged recombinant protein immunogen using ab61273 VDAC / Porin antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa.
  • Overlay histogram showing HepG2 cells stained with ab61273 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab61273, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-VDAC1/Porin antibody (ab61273) 使用論文

This product has been referenced in:
  • Wilding C  et al. ?-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway. PLoS One 9:e90737 (2014). Read more (PubMed: 24595072) »
  • Laafi J  et al. Pro-oxidant effect of ALA is implicated in mitochondrial dysfunction of HepG2 cells. Biochimie 106:157-66 (2014). Read more (PubMed: 25220386) »

See all 2 Publications for this product

Product Wall

Thank you for the information regarding the efficacy of the secondary antibody.
I will be happy to send ab14734 as a free-of-charge replacement for ab61273 now, if you like. You will still need to pay for ab61273, if you have not done so already. ...

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Thank you for troubleshooting our complaint form. Your protocol looks very good, and you should be able to detect VDAC with these conditions.

My one concern was the affinity-purified secondary from Jackson Lab, that it was isotype subclass-s...

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Thank you for following up with me, and I'm sorry to hear that the second vial also did not work well.

I am happy to send you a vial of ab14734, and this should arrive early next week on the order ***.

Please keep me updated about t...

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Thank you for your call today, and I am sorry for the trouble with this antibody.

As we discussed, I'm sending a free of charge vial of ab15895 on the order ***. This antibody is in stock and should arrive tomorrow.

Please keep me u...

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Thank you for your enquiry.
Currently, this antibody (ab34726) is out of stock. However, the good news is that we have some alternative products against the same target protein (VDAC/Porin). I do not know in which species/application your customer...

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Thank you for your reply. I am sending a free of charge vial of ab61273 from a new lot on the order ***. This product is in stockand it should arrive within a couple of days. Please keep me updated of the results using this replacement, and let me know...

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Thank you for contacting us and letting us know about the trouble with this vial of ab61273. I have looked through our records, and we haven't received any other reports of a similar problem with the lot that you currently have (I found your order and ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"