Anti-VDAC1/Porin 抗体 (ab34726)
Key features and details
- Rabbit polyclonal to VDAC1/Porin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-VDAC1/Porin antibody
VDAC1/Porin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VDAC1/Porin -
由来種
Rabbit -
特異性
Cross reactivity will occur with all forms of VDACs including VDAC1, VDAC2 (4 isoforms) and VDAC3(2 isoforms). This broad reactivity makes ab34726 useful as a loading control (mitochondrial). -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rabbit, Cow, Pig, Zebrafish -
免疫原
Synthetic peptide corresponding to Human VDAC1/Porin aa 185-197.
Sequence:NDGTEFGGSIYQK
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was affinity purified from monospecific antiserum. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab34726の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. -
組織特異性
Heart, liver and skeletal muscle. -
配列類似性
Belongs to the eukaryotic mitochondrial porin family. -
ドメイン
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity. -
細胞内局在
Mitochondrion outer membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 282119 Cow
- Entrez Gene: 7416 Human
- Entrez Gene: 22333 Mouse
- Entrez Gene: 397010 Pig
- Entrez Gene: 100008751 Rabbit
- Entrez Gene: 334582 Zebrafish
- Omim: 604492 Human
- SwissProt: P45879 Cow
see all -
別名
- N2441 antibody
- OMP2 antibody
- POR1 antibody
see all
画像
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IHC image of ab34726 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34726, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (59)
ab34726 は 59 報の論文で使用されています。
- Pinho SA et al. Mitochondrial and metabolic remodelling in human skin fibroblasts in response to glucose availability. FEBS J 289:5198-5217 (2022). PubMed: 35213938
- Sawicka E et al. The Influence of Interaction between Cadmium with 17β-Estradiol, 2-Methoxyestradiol and 16α-Hydroxyestrone on Viability and p-Glycoprotein in Ovarian Cancer Cell Line. Int J Mol Sci 23:N/A (2022). PubMed: 35269769
- Wang Q et al. Subsarcolemmal and cytoplasmic p62 positivity and rimmed vacuoles are distinctive for PLIN4-myopathy. Ann Clin Transl Neurol 9:1813-1819 (2022). PubMed: 36151849
- Wastag M et al. Celastrol and Rhynchophylline in the mitigation of simulated muscle atrophy under in vitro. Saudi Pharm J 30:1387-1395 (2022). PubMed: 36387339
- Latina V et al. Systemic delivery of a specific antibody targeting the pathological N-terminal truncated tau peptide reduces retinal degeneration in a mouse model of Alzheimer's Disease. Acta Neuropathol Commun 9:38 (2021). PubMed: 33750467