製品の概要

  • 製品名Anti-VCP antibody [Hs-14] (FITC)
    VCP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Hs-14] to VCP (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Tissue, cells or virus corresponding to Human VCP. Freshly ejaculated human sperm washed in PBS & extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and then used for immunization.

  • ポジティブ・コントロール
    • Sperm cells.
  • 特記事項

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報Flow Cyt: Use 20µl for 100µl cell suspension.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). Regulates E3 ubiquitin-protein ligase activity of RNF19A.
    • 関連疾患Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) [MIM:167320]; also known as muscular dystrophy, limb-girdle, with Paget disease of bone or pagetoid amyotrophic lateral sclerosis or pagetoid neuroskeletal syndrome or lower motor neuron degeneration with Paget-like bone disease. IBMPFD features adult-onset proximal and distal muscle weakness (clinically resembling limb girdle muscular dystrophy), early-onset Paget disease of bone in most cases and premature frontotemporal dementia.
    • 配列類似性Belongs to the AAA ATPase family.
    • 翻訳後修飾Phosphorylated by tyrosine kinases in response to T-cell antigen receptor activation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      ISGylated.
    • 細胞内局在Cytoplasm > cytosol. Nucleus. Present in the neuronal hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 15S Mg(2+) ATPase p97 subunit antibody
      • 15S Mg(2+)-ATPase p97 subunit antibody
      • ALS14 antibody
      • ATPase p97 antibody
      • CDC48 antibody
      • IBMPFD antibody
      • MGC131997 antibody
      • MGC148092 antibody
      • MGC8560 antibody
      • p97 antibody
      • TER ATPase antibody
      • TERA antibody
      • TERA_HUMAN antibody
      • Transitional endoplasmic reticulum ATPase antibody
      • Valosin containing protein antibody
      • Valosin-containing protein antibody
      • VCP antibody
      • Yeast Cdc48p homolog antibody
      see all

    Anti-VCP antibody [Hs-14] (FITC) (ab77238) 使用論文

    This product has been referenced in:
    • Peknicova J  et al. Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis. Am J Reprod Immunol 53:42-9 (2005). Read more (PubMed: 15667524) »
    • Chládek D  et al. [Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization] Ceska Gynekol 65:28-32 (2000). Read more (PubMed: 10750294) »

    See all 2 Publications for this product

    Product Wall

    The concentration is 190 ug/ml.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"