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Synthetic peptide corresponding to Human VCP aa 792-806.
Our Abpromise guarantee covers the use of ab11433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|ELISA||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ICC/IF||1/20 - 1/200.|
|WB||1/2000. Detects a band of approximately 97 kDa.Can be blocked with Mouse VCP peptide (ab39788).|
Image courtesy of an anonymous Abreview.
Abbreviations in the figure:
P : Permeabilized;
US Unstained, Red Peak;
IgG Rb: IgG Mouse (Isotype Control), Blue Peak;
VCP: Green Peak.
Western blot of VCP from CA46 cell lysate using ab11433.
ab11433 staining VCP in Human H1299 cells by Immunocytochemistry/ Immunofluorescence. The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100 and were blocked in 10% serum at 25°C for 1 hour. Primary antibody was used at 1/1000 dilution (PBS) and incubated with sample for 1 hour at 37°C. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG was used as secondary at 1/1000 dilution. Image shows green color staining with ab11433 and nuclei were stained blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"