製品の概要

  • 製品名Anti-VCAM1 antibody [M/K-2] (Phycoerythrin)
    VCAM1 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [M/K-2] to VCAM1 (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性ab25452 recognises CD106/VCAM1 (100 kDa)
  • アプリケーション適用あり: Flow Cyt, ICC/IF, Blocking, Inhibition Assay, IPmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • 特記事項


    The M/K-2 monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long term cardiac graft survival. It immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under nonreducing conditions.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.2µg for 106 cells. ab154449-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.

ab25452 blocks lymphocyte adhesion in Whitlock Witte long term bone marrow cultures.

Inhibition Assay Use at an assay dependent concentration.

ab25452 inhibits lymphopoiesis in Whitlock Witte cultures.

IP Use at an assay dependent concentration.

ab25452 immunoprecipitates a peptide that gives a single band on SDS PAGE gels with an apparent Mr of 100 kDa under reducing conditions and 92 kDa under nonreducing conditions.

ターゲット情報

  • 機能Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • 組織特異性Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • 配列類似性Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • ドメインEither the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • 翻訳後修飾Sialoglycoprotein.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

Anti-VCAM1 antibody [M/K-2] (Phycoerythrin) 画像

  • Flow cytometry analysis staining VCAM1 in mouse bone marrow cells using ab25452 at a dilution of 0.1 μg/106 cells.

Anti-VCAM1 antibody [M/K-2] (Phycoerythrin) (ab25452) 使用論文

This product has been referenced in:
  • Olme CH  et al. Live cell detection of chromosome 2 deletion and Sfpi1/PU1 loss in radiation-induced mouse acute myeloid leukaemia. Leuk Res 37:1374-82 (2013). Flow Cyt ; Mouse . Read more (PubMed: 23806234) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"