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The details of the immunogen for this antibody are not available.
The M/K-2 monoclonal antibody has been used in transplant studies to suppress cardiac rejection and induce long term cardiac graft survival. It immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent Mr of ~100 kDa under reducing conditions and 92 kDa under nonreducing conditions.
Our Abpromise guarantee covers the use of ab25452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells. ab154449-Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|Blocking||Use at an assay dependent concentration.
ab25452 blocks lymphocyte adhesion in Whitlock Witte long term bone marrow cultures.
|Inhibition Assay||Use at an assay dependent concentration.
ab25452 inhibits lymphopoiesis in Whitlock Witte cultures.
|IP||Use at an assay dependent concentration.
ab25452 immunoprecipitates a peptide that gives a single band on SDS PAGE gels with an apparent Mr of 100 kDa under reducing conditions and 92 kDa under nonreducing conditions.
Flow cytometry analysis staining VCAM1 in mouse bone marrow cells using ab25452 at a dilution of 0.1 μg/106 cells.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"