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Our Abpromise guarantee covers the use of ab78712 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 20 µg/ml. Predicted molecular weight: 81 kDa.|
|ICC||Use a concentration of 5 - 10 µg/ml.|
|IHC (Methanol fixed)||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution. Fresh frozen, acetone or -20C methanol fixed tissues are recommended. Traditional 4% PFA typically does not work well. Other fixatives have not been tested.|