Anti-VCAM1 抗体 [1G11B1] (Biotin) (ab7224)

製品の概要

  • 製品名
    Anti-VCAM1 antibody [1G11B1] (Biotin)
    VCAM1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1G11B1] to VCAM1 (Biotin)
  • 標識
    Biotin
  • アプリケーション
    適用あり: ICC, IP, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein corresponding to VCAM1.

  • エピトープ
    Epitope is unknown. This clone is also known as 1.G11B1 and 1G11.B1
  • ポジティブ・コントロール
    • Lymph nodes can be used as positive control. As negative control it is advised to use as first antibody a control murine IgG1 antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab7224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
  • 組織特異性
    Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue.
  • 配列類似性
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
  • ドメイン
    Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.
  • 翻訳後修飾
    Sialoglycoprotein.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CD106 antibody
    • CD106 Antigen antibody
    • INCAM 100 antibody
    • INCAM-100 antibody
    • L1CAM antibody
    • MGC99561 antibody
    • V-CAM 1 antibody
    • Vascular Cell Adhesion Molecule 1 antibody
    • Vascular cell adhesion protein 1 antibody
    • VCAM 1 antibody
    • VCAM-1 antibody
    • VCAM1 antibody
    • VCAM1_HUMAN antibody
    see all

画像

  • ab7224 staining VCAM1 in Human pluripotent stem cell derived cardiomyocytes by Flow Cytometry. Cells were cultured in a monolayer with PBS + 2% FBS. The sample was incubated with the primary antibody (1/100 in PBS and 2% FBS) for 30 minutes at 4°C. A Biotin-conjugated mouse IgG (1/1000) was used as the secondary antibody.

    Gating Strategy: Negative control population of pluripotent stem cells.

参考文献

This product has been referenced in:
  • Skelton RJ  et al. SIRPA, VCAM1 and CD34 identify discrete lineages during early human cardiovascular development. Stem Cell Res 13:172-9 (2014). Read more (PubMed: 24968096) »
  • Elliott DA  et al. NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes. Nat Methods 8:1037-40 (2011). Flow Cyt ; Human . Read more (PubMed: 22020065) »

See all 2 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocytes)
Permeabilization
No
Gating Strategy
Negative control population of pluripotent stem cells
Specification
Human pluripotent stem cell derived cardiomyocytes
Preparation
Cell harvesting/tissue preparation method: TrypLE treatment of cell monolayer
Sample buffer: PBS + 2% FBS
Username

Abcam user community

Verified customer

投稿 Aug 02 2016

Thank you for your enquiry. I am sorry you had trouble getting through to us. This antibody has not yet been tested in ELISA< so I cannot give you a recommended dilution. However, s general recommendation of a starting dilution for a purified anti...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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