製品の概要

  • 製品名Anti-VAV3 antibody [EP1130Y]
    VAV3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1130Y] to VAV3
  • アプリケーション適用あり: WB, IP, Flow Cyt, ICC/IFmore details
    適用なし: IHC-P
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-term of human Vav3.

  • ポジティブ・コントロール
    • Jurkat cell lysate; permeabalized Jurkat cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-VAV3 antibody (Alexa Fluor® 647) [EP1130Y] (ab206236)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IP 1/50.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration. PubMed: 23566222

Methanol fixed cells

  • 追加情報Is unsuitable for IHC-P.
  • ターゲット情報

    • 機能Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
    • 配列類似性Contains 1 CH (calponin-homology) domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ40431 antibody
      • Guanine nucleotide exchange factor VAV3 antibody
      • Protein vav 3 antibody
      • Protein vav3 antibody
      • RGD1565941 antibody
      • VAV 3 antibody
      • Vav 3 guanine nucleotide exchange factor antibody
      • VAV 3 oncogene antibody
      • VAV 3 protein antibody
      • VAV-3 antibody
      • Vav3 antibody
      • VAV3 oncogene antibody
      • VAV3 protein antibody
      • VAV3_HUMAN antibody
      see all

    Anti-VAV3 antibody [EP1130Y] 画像

    • Anti-VAV3 antibody [EP1130Y] (ab52938) at 1/100000 dilution + Jurkat cell lysate at 10 µg

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 98 kDa
      Observed band size : ~98 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized Jurkat cells using ab52938 (red) or a rabbit IgG (negative) (green).

    Anti-VAV3 antibody [EP1130Y] (ab52938) 使用論文

    This product has been referenced in:
    • Aguilar H  et al. VAV3 mediates resistance to breast cancer endocrine therapy. Breast Cancer Res 16:R53 (2014). Mouse . Read more (PubMed: 24886537) »
    • Nomura T  et al. Targeting the Vav3 oncogene enhances docetaxel-induced apoptosis through the inhibition of androgen receptor phosphorylation in LNCaP prostate cancer cells under chronic hypoxia. Mol Cancer 12:27 (2013). ICC/IF ; Human . Read more (PubMed: 23566222) »

    See all 3 Publications for this product

    Product Wall

    Thanks for the reply. I am a little stumped as to why you are having these issues. I can only think of 2 things that may help (and I am sure you have already tried them): 1 - One reason for multiply bands is that there is not enough protease inhibitor ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"