The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
Use at an assay dependent concentration.
1/500 - 1/1000.
Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
Western blot - VAV2 (phospho Y142) antibody (ab73707)
All lanes : Anti-VAV2 (phospho Y142) antibody (ab73707) at 1/500 dilution
Lane 1 : Extracts from K562 cells treated with TNF (200ng/ml, 30mins) Lane 2 : Extracts from Hela cells treated with TNF (200ng/ml, 30mins) Lane 3 : Extracts from K562 cells treated with TNF (200ng/ml, 30mins) plus 5µg immunizing phosphopeptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 101 kDa Observed band size : 101 kDa