Anti-VAV2 抗体 [EP1067Y] (ab52640)
製品の概要
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製品名Anti-VAV2 antibody [EP1067Y]
VAV2 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EP1067Y] to VAV2
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由来種Rabbit
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特異性This antibody detects Vav2 phosphorylated on Tyr172 as well as unphosphorylated Vav2.
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アプリケーション適用あり: WB, IP, ICC, IHC-Pmore details
適用なし: Flow Cyt -
種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VAV2. The exact sequence is proprietary.
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ポジティブ･コントロール
- IHC-P: Human cervical carcinoma. WB: 293 cell lysate. Flow Cyt: HeLa cells. IP: HEK293 cell lysate.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EP1067Y
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アイソタイプIgG
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研究分野
関連製品
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Alternative Versions
アプリケーション
Our Abpromise guarantee covers the use of ab52640 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/20000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa). | |
| IP | Use at an assay dependent concentration. | |
| ICC | 1/100 - 1/250. | |
| IHC-P | Use at an assay dependent concentration. |
ターゲット情報
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機能Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
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組織特異性Widely expressed.
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配列類似性Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains. - Information by UniProt
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参照データベース
- Entrez Gene: 7410 Human
- Entrez Gene: 22325 Mouse
- Entrez Gene: 296603 Rat
- Omim: 600428 Human
- SwissProt: P52735 Human
- SwissProt: Q60992 Mouse
- Unigene: 369921 Human
- Unigene: 689325 Human
see all -
別名
- Guanine nucleotide exchange factor VAV2 antibody
- Oncogene VAV2 antibody
- Protein vav 2 antibody
see all
画像
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![Western blot - VAV2 antibody [EP1067Y] (ab52640)](http://a.abcam.com/ps/products/52/ab52640/Images/vav1.jpg)
Western blot - VAV2 antibody [EP1067Y] (ab52640)Anti-VAV2 antibody [EP1067Y] (ab52640) at 1/20000 dilution + 293 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 101 kDa
Observed band size: 101 kDa -
![Flow Cytometry - Anti-VAV2 antibody [EP1067Y] (ab52640)](http://a.abcam.com/ps/products/52/ab52640/Images/ab52640-4-ab52640FC1.jpg)
Flow Cytometry - Anti-VAV2 antibody [EP1067Y] (ab52640)Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling VAV2 (red) with ab52640 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAV2 antibody [EP1067Y] (ab52640)](http://a.abcam.com/ps/products/52/ab52640/Images/vav2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAV2 antibody [EP1067Y] (ab52640)Human cervical carcinoma stained with ab52640 at 1/50 - 1/100 dilution -
![Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] (ab52640)](http://a.abcam.com/ps/products/52/ab52640/Images/VAV2-Primary-antibodies-ab52640-3.jpg)
Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] (ab52640)VAV2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Rabbit monoclonal to VAV2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52640.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 100kDa; VAV2.
参考文献
This product has been referenced in:
- Jones MC et al. Paxillin kinase linker (PKL) regulates Vav2 signaling during cell spreading and migration. Mol Biol Cell 24:1882-94 (2013). Human . Read more (PubMed: 23615439) »
- Yin S et al. Vav1-phospholipase C-?1 (Vav1-PLC-?1) pathway initiated by T cell antigen receptor (TCR?d) activation is required to overcome inhibition by ubiquitin ligase Cbl-b during ?dT cell cytotoxicity. J Biol Chem 288:26448-62 (2013). Read more (PubMed: 23897818) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"