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RabMAb

Anti-VAV2 抗体 [EP1067Y] (ab52640)

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製品の概要

  • 製品名Anti-VAV2 antibody [EP1067Y]
    VAV2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1067Y] to VAV2
  • 特異性This antibody detects Vav2 phosphorylated on Tyr172 as well as unphosphorylated Vav2.
  • アプリケーション適用あり: WB, IP, ICC, IHC-Pmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VAV2. The exact sequence is proprietary.

  • ポジティブ･コントロール
    • IHC-P: Human cervical carcinoma. WB: 293 cell lysate. Flow Cyt: HeLa cells. IP: HEK293 cell lysate.
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/20000. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
IP Use at an assay dependent concentration.
ICC 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.
  • 追加情報Is unsuitable for Flow Cyt.

    • ターゲット情報

    • 機能Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitement by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly.
    • 組織特異性Widely expressed.
    • 配列類似性Contains 1 CH (calponin-homology) domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 1 PH domain.
      Contains 1 phorbol-ester/DAG-type zinc finger.
      Contains 1 SH2 domain.
      Contains 2 SH3 domains.
    • Information by UniProt
    • 参照データベース
      see all
    • 別名
      • Guanine nucleotide exchange factor VAV2 antibody
      • Oncogene VAV2 antibody
      • Protein vav 2 antibody
      • VAV 2 antibody
      • Vav 2 oncogene antibody
      • VAV-2 antibody
      • VAV2 antibody
      • VAV2 oncogene antibody
      • VAV2_HUMAN antibody
      see all

    Anti-VAV2 antibody [EP1067Y] 画像

    • Western blot - VAV2 antibody [EP1067Y] (ab52640)
      Western blot - VAV2 antibody [EP1067Y] (ab52640)
      Anti-VAV2 antibody [EP1067Y] (ab52640) at 1/20000 dilution + 293 cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 101 kDa
      Observed band size : 101 kDa
    • Flow Cytometry - Anti-VAV2 antibody [EP1067Y] (ab52640)
      Flow Cytometry - Anti-VAV2 antibody [EP1067Y] (ab52640)

      Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling VAV2 (red) with ab52640 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAV2 antibody [EP1067Y] (ab52640)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAV2 antibody [EP1067Y] (ab52640)
      Human cervical carcinoma stained with ab52640 at 1/50 - 1/100 dilution
    • Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] (ab52640)
      Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] (ab52640)
      VAV2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Rabbit monoclonal to VAV2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
      The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52640.
      Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
      Band: 100kDa; VAV2.

    Anti-VAV2 antibody [EP1067Y] (ab52640) 使用論文

    This product has been referenced in:
    • Jones MC  et al. Paxillin kinase linker (PKL) regulates Vav2 signaling during cell spreading and migration. Mol Biol Cell 24:1882-94 (2013). Human . Read more (PubMed: 23615439) »
    • Yin S  et al. Vav1-phospholipase C-?1 (Vav1-PLC-?1) pathway initiated by T cell antigen receptor (TCR?d) activation is required to overcome inhibition by ubiquitin ligase Cbl-b during ?dT cell cytotoxicity. J Biol Chem 288:26448-62 (2013). Read more (PubMed: 23897818) »

    See all 9 Publications for this product

    ab52640 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab52640 の abreview を投稿する ab52640 に関する問い合わせをする

    3 Abreviews or Q&A

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    Western blot abreview for Anti-VAV2 antibody [EP1067Y]

     Excellent
    Abreviews
    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
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    Username

    Abcam user community

    Verified customer

    投稿 Dec 03 2014

    Western blot abreview for Anti-VAV2 antibody [EP1067Y]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (human and mouse cell lines)
    Loading amount 50 µg
    Specification human and mouse cell lines
    Gel Running Conditions Reduced Denaturing (8)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More
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    Abcam user community

    Verified customer

    投稿 Jul 28 2010

    Western blot abreview for Anti-VAV2 antibody [EP1067Y]

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HT29 cells)
    Loading amount 20 µg
    Specification HT29 cells
    Treatment EPA for 24 hrs
    Gel Running Conditions Reduced Denaturing (10% bis tris gel)
    Blocking step Thermo scientific superblock buffer in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
    Read More
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    Dr. Joanne Doleman

    Verified customer

    投稿 Oct 22 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"