Anti-Vav proteins (phospho Y174) 抗体 (ab47282)

製品の概要

  • 製品名Anti-Vav proteins (phospho Y174) antibody
    Vav proteins 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Vav proteins (phospho Y174)
  • 特異性This antibody is specific for Vav protein, only when phosphorylated at tyrosine 174.
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthesized phosphopeptide derived from human Vav proteins around the phosphorylation site of tyrosine 174 (E-I-YP-E-D)

  • ポジティブ・コントロール
    • Jurkat cell extracts

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab47282 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution. See Abreview.
WB 1/500 - 1/1000. Detects a band of approximately 118 kDa.
ELISA 1/10000.

ターゲット情報

  • 関連性The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels. As a signalling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein. Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response.
  • 細胞内局在Cytoplasmic and Plasma membrane VAV1, Cytoplasm - VAV2 & VAV3
  • 参照データベース
  • 別名
    • 2810040F13Rik antibody
    • A530094I06Rik antibody
    • AA986410 antibody
    • AI847175 antibody
    • FLJ40431 antibody
    • GDP/GTP exchange factor VAV2 antibody
    • Guanine nucleotide exchange factor VAV2 antibody
    • Guanine nucleotide exchange factor VAV3 antibody
    • MGC27838 antibody
    • Oncogene VAV antibody
    • Oncogene VAV1 antibody
    • Oncogene VAV2 antibody
    • Oncogene VAV3 antibody
    • p95 antibody
    • p95vav antibody
    • Protein vav 1 antibody
    • Protein vav 2 antibody
    • Protein vav 3 antibody
    • Proto oncogene vav antibody
    • RGD1565941 antibody
    • VAV antibody
    • vav 1 guanine nucleotide exchange factor antibody
    • Vav 1 oncogene antibody
    • vav 2 guanine nucleotide exchange factor antibody
    • Vav 2 oncogene antibody
    • vav 3 guanine nucleotide exchange factor antibody
    • Vav 3 oncogene antibody
    • vav-T antibody
    • VAV1 oncogene antibody
    • VAV2 oncogene antibody
    • VAV3 oncogene antibody
    see all

Anti-Vav proteins (phospho Y174) antibody 画像

  • All lanes : Anti-Vav proteins (phospho Y174) antibody (ab47282) at 1/500 dilution

    Lane 1 : Extrats from Jurkat cells treated with UV
    Lane 2 : Extrats from Jurkat cells untreated

    Lysates/proteins at 30 µg per lane.

Anti-Vav proteins (phospho Y174) antibody (ab47282) 使用論文

This product has been referenced in:
  • Kajla S  et al. A crucial role for Nox 1 in redox-dependent regulation of Wnt-ß-catenin signaling. FASEB J : (2012). Read more (PubMed: 22278940) »
  • Puschmann TB & Turnley AM Eph receptor tyrosine kinases regulate astrocyte cytoskeletal rearrangement and focal adhesion formation. J Neurochem 113:881-94 (2010). WB ; Mouse . Read more (PubMed: 20202079) »

See all 5 Publications for this product

Product Wall


I would not worry about results where the observed molecular weights are 10 or even 15% above the expected molecular weights. As explained in our http://www.abcam.com/protocols/western-blot-faq, this is very common and explained by the post-transl...

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Thank you for your phone call. As I mentioned, because these are phospho-specific antibodies, we recommend using BSA as a blocking agent instead of milk. If this does not help to improve your results, please let me knowyour original order or PO numbera...

Read More

The difference between these two products is that ab76225 is a rabbit monoclonal antibody while ab47282 is a rabbit polyclonal antibody. Both of these antibodies were raised against the corresponding residues around the phophorylated site at the 174 am...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (platelet)
Loading amount 15 µg
Specification platelet
Treatment MnCl2/PP2 15 min
Gel Running Conditions Reduced Denaturing (7.5 % gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
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Verified customer

投稿 May 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Platelet)
Specification Platelet
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 May 26 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"