製品の概要

  • 製品名Anti-VASP antibody [1H8]
    VASP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1H8] to VASP
  • アプリケーション適用あり: WB, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human VASP produced in HEK293T cells (NP_003361).

  • ポジティブ・コントロール
    • HEK293T cell lysate transfected with pCMV6-ENTRY VASP cDNA; COS7 cells transiently transfected by pCMV6-ENTRY VASP; HepG2, HeLa, MCF7, HT29 and A549 cell extracts; HEK293T cells transfected with pCMV6-ENTRY VASP overexpress plasmid.
  • 特記事項Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 48% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)ab114029 is purified from Mouse ascites fluid by affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名1H8
  • アイソタイプIgG1
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab114029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Predicted molecular weight: 40 kDa.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

ターゲット情報

  • 機能Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.
  • 組織特異性Highly expressed in platelets.
  • 配列類似性Belongs to the Ena/VASP family.
    Contains 1 WH1 domain.
  • ドメインThe EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
    The WH1 domain mediates interaction with XIRP1.
  • 翻訳後修飾Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.
  • 細胞内局在Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Vasodilator stimulated phosphoprotein antibody
    • Vasodilator-stimulated phosphoprotein antibody
    • VASP antibody
    • VASP_HUMAN antibody

Anti-VASP antibody [1H8] 画像

  • All lanes : Anti-VASP antibody [1H8] (ab114029) at 1/500 dilution

    Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY VASP cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 40 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203544)
  • All lanes : Anti-VASP antibody [1H8] (ab114029) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 40 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203544)
  • ab114029 at 1/100 dilution staining VASP in COS7 cells transiently transfected with pCMV6-ENTRY VASP by Immunofluorescence.
  • ab114029 at 1/100 dilution staining VASP in HEK293T cells transfected with either pCMV6-ENTRY VASP overexpress plasmid (Red) or empty vector control plasmid (Blue) by flow cytometry.

Anti-VASP antibody [1H8] (ab114029) 使用論文

ab114029 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"