Anti-VASP 抗体 [OTI1H8] (ab114029)
Key features and details
- Mouse monoclonal [OTI1H8] to VASP
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-VASP antibody [OTI1H8]
VASP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OTI1H8] to VASP -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IFmore details -
種交差性
交差種: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human VASP aa 1 to the C-terminus. Produced in HEK-293T cells. NP_003361
Database link: P50552 -
ポジティブ・コントロール
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY VASP cDNA; HepG2, HeLa, MCF7, HT-29 and A549 cell extracts; ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY VASP; Flow Cyt (Intra): HEK-293T cells transfected with pCMV6-ENTRY VASP overexpress plasmid.
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特記事項
The clone number has been updated from 1H8 to OTI1H8, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Purified from cell culture supernatant by affinity chromatography -
ポリ/モノ
モノクローナル -
クローン名
OTI1H8 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab114029の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
1/500 - 1/2000. Predicted molecular weight: 40 kDa.
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ICC/IF |
1/100.
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特記事項 |
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Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 40 kDa. |
ICC/IF
1/100. |
ターゲット情報
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機能
Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. -
組織特異性
Highly expressed in platelets. -
配列類似性
Belongs to the Ena/VASP family.
Contains 1 WH1 domain. -
ドメイン
The EVH2 domain is comprised of 3 regions. Block A is a thymosin-like domain required for G-actin binding. The KLKR motif within this block is essential for the G-actin binding and for actin polymerization. Block B is required for F-actin binding and subcellular location, and Block C for tetramerization.
The WH1 domain mediates interaction with XIRP1. -
翻訳後修飾
Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239. -
細胞内局在
Cytoplasm. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Cell projection > lamellipodium membrane. Cell projection > filopodium membrane. Targeted to stress fibers and focal adhesions through interaction with a number of proteins including MRL family members. Localizes to the plasma membrane in protruding lamellipodia and filopodial tips. Stimulation by thrombin or PMA, also translocates VASP to focal adhesions. Localized along the sides of actin filaments throughout the peripheral cytoplasm under basal conditions. - Information by UniProt
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参照データベース
- Entrez Gene: 7408 Human
- Omim: 601703 Human
- SwissProt: P50552 Human
- Unigene: 515469 Human
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別名
- Vasodilator stimulated phosphoprotein antibody
- Vasodilator-stimulated phosphoprotein antibody
- VASP antibody
- VASP_HUMAN antibody
画像
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All lanes : Anti-VASP antibody [OTI1H8] (ab114029) at 1/500 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) cell extract
Lane 4 : A549 (Human lung carcinoma cell line) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extract
Lane 7 : MDCK (Canine kidney cell line) cell extract
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extract
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 40 kDa -
ab114029 at 1/100 dilution staining VASP in COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY VASP by Immunofluorescence.
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ab114029 at 1/100 dilution staining VASP in HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY VASP overexpress plasmid (Red) or empty vector control plasmid (Blue) by Flow Cytometry (Intracellular).
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All lanes : Anti-VASP antibody [OTI1H8] (ab114029) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY VASP cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 40 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab114029 は論文での使用が確認できていません。