Anti-AVPR1B 抗体 (ab104365)
Key features and details
- Rabbit polyclonal to AVPR1B
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-AVPR1B antibody
AVPR1B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to AVPR1B -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Mouse AVPR1B aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab124133) -
ポジティブ・コントロール
- This antibody gave a positive signal in NIH3T3 whole cell lysate as well as the following tissue lysates: E10 Mouse Embryo Brain; Mouse Forebrain; Human Brain Membrane
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104365の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 46 kDa). |
ターゲット情報
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機能
Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 553 Human
- Entrez Gene: 26361 Mouse
- Omim: 600264 Human
- SwissProt: P47901 Human
- SwissProt: Q9WU02 Mouse
- Unigene: 1372 Human
- Unigene: 89986 Mouse
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別名
- Antidiuretic hormone receptor 1b antibody
- Antidiuretic hormone, receptor for, V1B antibody
- Arginine vasopressin receptor 1B antibody
see all
画像
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All lanes : Anti-AVPR1B antibody (ab104365) at 1 µg/ml
Lane 1 : E10 Mouse Embryo Brain Tissue Lysate
Lane 2 : Forebrain (Mouse) Tissue Lysate
Lane 3 : Human brain normal tissue lysate - membrane extract (ab29456)
Lane 4 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 5 : E10 Mouse Embryo Brain Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 6 : Forebrain (Mouse) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 7 : Human brain normal tissue lysate - membrane extract (ab29456) with Immunising peptide at 1 µg/ml
Lane 8 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 102 kDa, 22 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
VR1b contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab104365 は 1 報の論文で使用されています。
- Dannenhoffer CA et al. Oxytocin and vasopressin modulation of social anxiety following adolescent intermittent ethanol exposure. Psychopharmacology (Berl) 235:3065-3077 (2018). PubMed: 30141056