製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Vasopressin
  • アプリケーション
    適用あり: IHC-FoFr, IHC-Frmore details
  • 種交差性
    交差種: Rat, Pig
  • 免疫原

    Full length native protein (purified)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab39363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration.
IHC-Fr 1/500 - 1/5000.

ターゲット情報

  • 関連性
    Vasopressin, also known as arginine vasopressin (AVP) or antidiuretic hormone (ADH), is a posterior pituitary hormone that is synthesised in the hypothalamus. Vasopressin is synthesised as a precursor protein that consists of arginine vasopressin and two associated proteins, neurophysin 2 and the glycopeptide copeptin. Vasopressin, together with its carrier protein neurophysin II, is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney and also causes vasoconstriction of the peripheral vessels. Vasopressin can also contract smooth muscle during parturition and lactation. It also plays a role in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in the vasopressin precursor cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI), which is characterised by persistant thirst, polydipsia and polyuria.
  • 細胞内局在
    Secreted
  • 参照データベース
  • 別名
    • ADH antibody
    • Antidiuretic hormone antibody
    • Arginine vasopressin neurophysin II antibody
    • ARVP antibody
    • AVP antibody
    • AVP NPII antibody
    • copeptin antibody
    • Vasopressin neurophysin II copeptin antibody
    • VP antibody
    see all

画像

  • Minipigs were deeply anesthetized with a combination of midazolam and ketamine, prior to transcardial perfusion with phosphate buffered 4% paraformaldehyde (pH 7.4). After perfusion, the brains were removed with special care taken to preserve the optic chiasm and the median eminence. Blocks of tissue containing the hypothalami were dissected, postfixed in the same fixative for 1 day and subsequently cryoprotected in 30% sucrose for 3–4 days, prior to freezing. 10 series of 40-mm thick coronal (6 animals), sagittal (1 animal), and horizontal (1 animal) cryostat sections were collected. Coronal sections for immunohistochemistry were maintained at -18°C as free-floating sections in a cryoprotectant poly-ethylene glycol solution for up to four weeks.
    Immunohistochemistry was performed using the avidin-biotin method. Accordingly, free-floating sections were first rinsed in Tris-buffered saline (TBS; 0.05 M; pH 7.4) for 15 minutes. Incubations with avidin (0.1%) and biotin (0.01%) were each of 10 minute duration and each procedure were followed by a 2 minute TBS-rinse. The sections were preincubated with 1% Triton X- 100 and 0.2% milk in TBS for 30 minutes, prior to incubation with the primary antibody, ab39363, for 72 hours at 4°C at a 1/100 dilution. The sections were then washed three times 15 minutes in TBS and 1% Triton X-100 prior to incubation for 1 hour at room temperature with the secondary anti-rabbit biotinylated antibody at a 1/400 with TBS that contained 1% Triton X- 100 and 0.2% milk. After a brief rinse in TBS, the endogenous peroxidase activity was blocked with a solution of 10 ml hydrogen peroxide, 10 ml methanol, and 80 ml TBS, for 10 minutes. The sections were then rinsed in TBS and 1% Triton for three times 15 minutes and incubated for 1 hour at room temperature with avidin-peroxidas diluted 1/400 in TBS that contained 1% Triton X-100 and 0.2% milk. The sections were then rinsed three times for 15 minutes in TBS and 1% Triton, prior to avidin-peroxidase visualization. The latter step consisted of incubation for 10 minutes in 10ml water in which one diaminobenzidine tablet had been dissolved and to which 10 ml of 35% H2O2 had been added. Finally, the sections were dehydrated, mounted and coverslipped with Depex.

    och: optic chiasm.

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参考文献

This product has been referenced in:

See all 8 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (brain (hypothalamus))
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
60 µg
Specification
brain (hypothalamus)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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Verified customer

投稿 Jul 04 2017

Thank you for your enquiry.

Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

In this instance, none of our Vaso...

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Thank you for your inquiry. Unfortunately, we are unable to track down the particular antibodies the authors used when it is not specifically mentioned in the paper. However, the following antibodies might have been used: ab39363: ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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