製品の概要

  • 製品名Anti-VASH1 antibody
    VASH1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to VASH1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: GGKKVAGGGS SGATPTSAAA TAPSGVRRLE TSEGTSAQRD EEPEEEGEED LRDGGVPFFV NRGGLPVDEA TWERMWKHVA KIHPDGEKVA QRIRGATD, corresponding to amino acids 3-100 of Human VASH1 (NP_055724) with a proprietary tag.

  • ポジティブ・コントロール
    • Tagged recombinant human VASH1 fragment (the immunogen). HepG2 cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab88435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 41 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity.
  • 組織特異性Preferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions.
  • 配列類似性Belongs to the vasohibin family.
  • 翻訳後修飾2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa) may be processed by proteolytic cleavage. The largest form (42 kDa) seems to be secreted and the other major form (63 kDa) seems to accumulate within the cells or pericellular milieu. Polypeptide consisting of Met-77 to Arg-318 may correspond to the 27 kDa form and that consisting of Met-77 to Val-365 may correspond to the 36 kDa form.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA1036 antibody
    • VASH antibody
    • VASH1 antibody
    • VASH1_HUMAN antibody
    • Vasohibin 1 antibody
    • Vasohibin-1 antibody
    see all

Anti-VASH1 antibody 画像

  • Anti-VASH1 antibody (ab88435) at 1 µg/ml + Tagged recombinant Human VASH1 fragment (the immunogen) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 41 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-VASH1 antibody (ab88435) at 1 µg/ml + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 41 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)

Anti-VASH1 antibody (ab88435) 使用論文

ab88435 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"