製品の概要

  • 製品名Anti-VAPA antibody
    VAPA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VAPA
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, Pig, Zebrafish
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 29 - 92 of Human VAPA.

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji or NIH3T3 cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.

ターゲット情報

  • 機能May play a role in vesicle trafficking.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
    Contains 1 MSP domain.
  • 細胞内局在Endoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 33 kDa Vamp-associated protein antibody
    • hVAP 33 antibody
    • MGC3745 antibody
    • OTTHUMP00000162362 antibody
    • OTTHUMP00000162363 antibody
    • VAMP (vesicle-associated membrane protein) associated protein A, 33kDa antibody
    • VAMP A antibody
    • VAMP associated protein A antibody
    • VAMP-A antibody
    • VAMP-associated protein A antibody
    • VAP 33 antibody
    • VAP A antibody
    • VAP-33 antibody
    • VAP-A antibody
    • VAP33 antibody
    • Vapa antibody
    • VAPA_HUMAN antibody
    • Vesicle associated membrane protein associated protein A antibody
    • Vesicle-associated membrane protein-associated protein A antibody
    see all

Anti-VAPA antibody 画像

  • IHC image of VAPA staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96584, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-VAPA antibody (ab96584) at 1/1000 dilution (12% SDS PAGE)

    Lane 1 : H1299 whole cell lysate
    Lane 2 : NIH3T3 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 27 kDa

Anti-VAPA antibody (ab96584) 使用論文

ab96584 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab96584.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"