製品の概要

製品の特性

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アプリケーション

Our Abpromise guarantee covers the use of ab3346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.

Used at a dilution of 1/1000 for 48 hrs incubation on mouse adult retina cells (see Abreview for further information).

IP Use at an assay dependent concentration.
WB Use a concentration of 0.3 µg/ml. Can be blocked with VAMP1 peptide (ab4957). By Western blot, this antibody detects a 15 kDa protein representing VAMP 1 from rat brain whole protein extract.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Involved in the targeting and/or fusion of transport vesicles to their target membrane.
  • 組織特異性Nervous system, skeletal muscle and adipose tissue.
  • 配列類似性Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • 細胞内局在Mitochondrion outer membrane; Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome and Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686H12131 antibody
    • SYB 1 antibody
    • SYB1 antibody
    • Synaptobrevin 1 antibody
    • Synaptobrevin-1 antibody
    • Synaptobrevin1 antibody
    • VAMP 1 antibody
    • VAMP-1 antibody
    • Vamp1 antibody
    • VAMP1_HUMAN antibody
    • Vesicle associated membrane protein 1 antibody
    • Vesicle associated membrane protein 1 synaptobrevin 1 antibody
    • Vesicle-associated membrane protein 1 antibody
    see all

Anti-VAMP1 antibody 画像

  • Western blot detection of VAMP1 on rat brain extract using ab3346.

  • ab3346 at a 1/1000 dilution staining mouse adult retina cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 48 hours. Bound antibody was detected using an Alexa Fluor ® 488 conjugated donkey anti rabbit antibody. Dilutions of 1/1000 through to 1/5000  were found to work.

    Staining in the outer plexiform layer was fainter than in the ganglion cell layer

    See Abreview

  • ab3346 (2µg/ml) staining VAMP1 in human cerebral cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and some membrane staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunofluorescence analysis of Huh7.5 cells transfected with GFP-VAMP1, staining VAMP1 with ab3346 at 1/250 dilution. An AlexaFluor®-conjugated IgG (1/1000) was used as the secondary antibody.

Anti-VAMP1 antibody (ab3346) 使用論文

This product has been referenced in:
  • Bate C  et al. Sialic Acid within the Glycosylphosphatidylinositol Anchor Targets the Cellular Prion Protein to Synapses. J Biol Chem 291:17093-101 (2016). Read more (PubMed: 27325697) »
  • Jiao SS  et al. Brain-derived neurotrophic factor protects against tau-related neurodegeneration of Alzheimer's disease. Transl Psychiatry 6:e907 (2016). IHC-FrFl, WB ; Mouse . Read more (PubMed: 27701410) »

See all 9 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Adult retina)
Specification Adult retina
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Jun 14 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"