製品の概要

  • 製品名
    Anti-Vaccinia Virus antibody
    Vaccinia Virus 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Vaccinia Virus
  • 特異性
    This antibody reacts with purified Virions. It does not react with uninfected cells.
  • アプリケーション
    適用あり: ELISA, WB, IHC-P, IHC-Fr, ICC/IFmore details
  • 種交差性
    Does not react with parainfluenza (1-3), RSV, Adeno, Influenza A and B or HSV (1). Reactive with the Lister, Wyeth, New York City and MVA strains of Vaccinia.
  • 免疫原

    Native virus, Lister strain.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 特記事項(精製)
    This antibody is greater than 95% pure.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab35219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/500 - 1/2000.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 25093734
IHC-P Use at an assay dependent concentration. PubMed: 25093734
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22615950

ターゲット情報

  • 関連性
    Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.
  • 別名
    • A27L antibody
    • Orthopoxvirus antibody

Anti-Vaccinia Virus antibody (ab35219) 使用論文

This product has been referenced in:
  • Larsen IV  et al. Both CD8+ and CD4+ T Cells Contribute to Corneal Clouding and Viral Clearance following Vaccinia Virus Infection in C57BL/6 Mice. J Virol 90:6557-72 (2016). IHC-P . Read more (PubMed: 27170749) »
  • Adelfinger M  et al. Preclinical Testing Oncolytic Vaccinia Virus Strain GLV-5b451 Expressing an Anti-VEGF Single-Chain Antibody for Canine Cancer Therapy. Viruses 7:4075-92 (2015). WB . Read more (PubMed: 26205404) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cross section of mouse eye, looked at cornea)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS EDTA pH 9
Permeabilization
No
Specification
Cross section of mouse eye, looked at cornea
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Ms. Inna Larsen

Verified customer

投稿 Dec 29 2015


Unfortunately, none of the anti-Vaccinia Virus antibodies we have has been tested for cross-reactivity with Western Reserve Strain.


ab117453 has been tested and cross-reacts with 3 other Vaccinia strains and also with distantly relat...

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Thank you for contacting us.

We only have two monoclonal antibodies against the vaccinia virus in our catalogue.

I would recommend using ab21098 - Mouse monoclonal [TV46] to Vaccinia Virus (http://www.abcam.com/ab21098) to recognise A...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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