The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/2000.
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
GKPIPNPLLGLDST epitope tag antibody
GKPIPNPLLGLDST tag antibody
Protein Rev antibody
Regulator of expression of viral proteins antibody
V5 epitope tag antibody
Immunocytochemistry/ Immunofluorescence - V5 tag antibody (FITC) (ab1274)Image from Adams JC et al, J Cell Sci. 2008 Mar 15;121(Pt 6):784-95. Epub 2008 Feb 19, Fig 2. DOI 10.1242/?jcs.021006
ab1274 staining V5 tag (FITC) in transfected COS-7 cells by Immunocytochemistry/ Immunofluorescence. Drosophila thrombospondins were expressed with V5-epitope tags and detected with ab1274. Thrombospondins are retained in the extracellular matrix of the COS-7 cells. Scale bar: 20 µm.
Francis O et al. Molecular Phylogeny of a RING E3 Ubiquitin Ligase, Conserved in Eukaryotic Cells and Dominated by Homologous Components, the Muskelin/RanBPM/CTLH Complex. PLoS One8:e75217 (2013).
Read more (PubMed: 24143168) »
Linka Y et al. The impact of TEL-AML1 (ETV6-RUNX1) expression in precursor B cells and implications for leukaemia using three different genome-wide screening methods. Blood Cancer J3:e151 (2013).
Read more (PubMed: 24121163) »