製品の概要

  • 製品名
    Anti-V5 tag antibody (FITC)
    V5 tag 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to V5 tag (FITC)
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 免疫原

    GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.

  • 特記事項

    Molar F/P ratio is 4.1:1

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: 0.2% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Antibodies were immunoaffinity purified using the peptide immobilized on a solid phase and conjugated to fluorescein isothiocyanate.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    unknown
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Can be blocked with V5 tag peptide (ab15829).
ELISA 1/5000 - 1/15000.
ICC/IF Use at an assay dependent concentration. PubMed: 21911501

ターゲット情報

  • 関連性
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • 別名
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Anti-V5 tag antibody (FITC) 画像

  • ab1209, staining V5 tag (green) in U373 cells by Immunocytochemistry/ Immunofluorescence.

    Cells were transfected with TRIL-V5 and stimulated with 25 µg/ml PIC for 0 (left) and 4 hours (right). Samples were fixed with formaldehyde and permeabilized using 0.2% Triton X-100 for 5 minutes before being incubated with primary antibody.

Anti-V5 tag antibody (FITC) (ab1209) 使用論文

This product has been referenced in:
  • Carpenter S  et al. Toll-like Receptor 3 (TLR3) Signaling Requires TLR4 Interactor with Leucine-rich Repeats (TRIL). J Biol Chem 286:38795-804 (2011). ICC/IF . Read more (PubMed: 21911501) »

See 1 Publication for this product

Product Wall

Thank you very much for your interest in ab1209. To our knowledge, ab1209 has not been tested in IF. Therefore, I can offer a discount off a future purchase if you buy ab1209 now, test it inIF and submit feedback to us in the form of an Abreview. It do...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (neuroblastoma cells)
Specification
neuroblastoma cells
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.1 % Triton, 0.2 % BSA-PBS 1X
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Min Kang

Verified customer

投稿 Aug 15 2011

Thank you for your enquiry. This product has an expiration date of 12 months. If you need any further assistance please do not hesitate to get back in touch.

Good results have been gained with this antibody up to 200ng/ml for western blotting. As a detection antibody in ELISA assays it could be used up to 50ng/ml. For your purposes a starting concentration of 2ug/ml is reccomended.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録