製品の概要

  • 製品名Anti-V5 tag antibody
    V5 tag 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to V5 tag
  • アプリケーション適用あり: ELISA, WB, ICC, IHC-Pmore details
  • 免疫原

    V5 tag (GKPIPNPLLGLDST) coupled to KLH

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use a concentration of 0.03 - 10 µg/ml. Coating: 2 - 10 ug/ml; Primary: 0.03 - 1 ug/ml
WB Use a concentration of 0.03 - 1 µg/ml. Colorimetric: 0.1 - 1 ug/ml; Chemiluminescent: 0.03 - 1 ug/ml
ICC Use a concentration of 0.5 - 5 µg/ml.
IHC-P Use at an assay dependent dilution.

ターゲット情報

  • 関連性The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • 別名
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Anti-V5 tag antibody 画像

  • A, cells expressing 315-HARE, 190-HARE, or EV alone were cultured in T-25 flasks until confluent, washed, scraped, and lysed. Cell lysates (100ug of protein) were immunoprecipitated (IP) with anti-Tyr(P) mAb and the immune complexes were subjected to 5% SDS-PAGE and Western blot (WB) analysis using anti-V5 pAb. Cells expressing 315-HARE (B) or 190-HARE (C) cDNA were grown until confluent, washed, incubated for 1 h in medium without serum, and then incubated with (HA) or without (Ctr) 5ug/ml HA for 20 min. The cells were lysed and 200 ug of total lysate protein was immunoprecipitated with anti-HARE mAb-30. Immune complexes were collected and subjected to 5% SDS-PAGE and Western analysis using anti- Tyr(P) mAb. The same blots in B and C were reprobed with anti-V5 Ab (D and E, respectively). Immunopurification using non-immune mouse IgG served as another control in B–E. The solid and open arrows indicate the positions of the 315-HARE and 190-HARE, respectively.
  • All lanes : Anti-V5 tag antibody (ab95038) at 0.4 µg/ml

    Lane 1 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
    Lane 2 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
    Lane 3 : E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg

    Developed using the ECL technique

    Exposure time : 30 seconds
  • Immunohistochemical analysis of murine mammary tumour tissue, expressing vector (left) or V5-tagged Cadm1 (right), staining V5 tag with ab95038.

Anti-V5 tag antibody (ab95038) 使用論文

This product has been referenced in:
  • Akhtar AA  et al. A transposon-mediated system for flexible control of transgene expression in stem and progenitor-derived lineages. Stem Cell Reports 4:323-31 (2015). WB, IHC ; Mouse . Read more (PubMed: 25702640) »
  • Breunig JJ  et al. Ets Factors Regulate Neural Stem Cell Depletion and Gliogenesis in Ras Pathway Glioma. Cell Rep 12:258-271 (2015). Mouse . Read more (PubMed: 26146073) »

See all 3 Publications for this product

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Thank you for your reply. I am very pleased to hear you would like to use ab95038 in IHC-P and send us an image of your results.

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Thank you for your reply.

I am very sorry to let you know that as the western blot images provided are from external testing, we have no further information to provide on this occasion. My apologies for the inconvenience in this case.
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Thank you for your recent telephone enquiry.

I am pleased to attach some WB images from ab95038. I hope these will be helpful to you. I am sorry there are currently no IHC-P images available. Please let me know before purchasing if you would...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"