Anti-V5 tag 抗体 (ab15828)
Key features and details
- Rabbit polyclonal to V5 tag
- Suitable for: ChIP, WB
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-V5 tag antibody
V5 tag 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to V5 tag -
由来種
Rabbit -
アプリケーション
適用あり: ChIP, WBmore details -
種交差性
交差種: Species independent -
免疫原
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ポジティブ・コントロール
- WB: Recombinant V5 tagged Groucho homolog. ChIP: A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15828の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ChIP |
Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads.
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WB | (1) |
Use a concentration of 0.5 µg/ml.
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特記事項 |
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ChIP
Use at an assay dependent concentration. ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. |
WB
Use a concentration of 0.5 µg/ml. |
ターゲット情報
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関連性
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
別名
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
画像
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A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a V5 or T7- tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the Abcam X-ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).
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All lanes : Anti-V5 tag antibody (ab15828) at 0.5 µg/ml
Lane 1 : Recombinant V5 tagged Groucho homolog
Lane 2 : Recombinant V5 tagged Groucho withV5 tag peptide (ab15829) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (49)
ab15828 は 49 報の論文で使用されています。
- Garipler G et al. The BTB transcription factors ZBTB11 and ZFP131 maintain pluripotency by repressing pro-differentiation genes. Cell Rep 38:110524 (2022). PubMed: 35294876
- Ito K et al. The oncogene-dependent resistance to reprogramming unveils cancer therapeutic targets. Cell Rep 39:110721 (2022). PubMed: 35476996
- Herre C et al. Expression of vimentin, TPI and MAT2A in human dermal microvascular endothelial cells during angiogenesis in vitro. PLoS One 17:e0266774 (2022). PubMed: 35482724
- Tavares M et al. JAZF1-SUZ12 dysregulates PRC2 function and gene expression during cell differentiation. Cell Rep 39:110889 (2022). PubMed: 35649353
- Fell CW et al. FIBCD1 is an endocytic GAG receptor associated with a novel neurodevelopmental disorder. EMBO Mol Med 14:e15829 (2022). PubMed: 35916241