Rabbit polyclonal to v-Myb + c-Myb (phospho S12)
ab78892 detects endogenous levels of v-Myb + c-Myb only when phosphorylated at serine 12.
Synthetic phosphopeptide derived from human v-Myb + c-Myb around the phosphorylation site of serine 12 (Y-S-S
Human heart tissue. Extracts from HeLa cells, treated with Hu (2nM, 24hours).
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 72 kDa.
1/50 - 1/100.
Transcriptional activator; DNA binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells.
C myb antibody
C myb protein antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - v-Myb + c-Myb (phospho S12) antibody (ab78892)
Immunohistochemistry analysis of paraffin-embedded human heart tissue using ab78892 at 1/50 dilution in the absence or presence of the immunising phosphopeptide.
Western blot - v-Myb + c-Myb (phospho S12) antibody (ab78892)
All lanes : Anti-v-Myb + c-Myb (phospho S12) antibody (ab78892) at 1/500 dilution Lane 1 : Extracts from HeLa cells treated with Hu (2nM, 24hours) Lane 2 : Extracts from HeLa cells treated with Hu (2nM, 24hours) with immunising phosphopeptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size: 72 kDa Observed band size: 72 kDa
has not yet been referenced specifically in any publications.
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